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  5. Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit

Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit

Numéro de catalogue. A36227
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Numéro de catalogue. No. of Reactions
A36227 50 Preps
A35892 25 Preps
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Numéro de catalogue. A36227 Fournisseur Invitrogen™ Code fournisseur A36227
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Description

The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/μg) plasmid DNA in about 30 minutes.

The simple precipitation-free protocol yields up to 0.4 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.

  • Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
  • Low endotoxin plasmid—the resulting plasmid contains less than 1 EU/μg endotoxins
  • High yields—isolate up to 0.4 mg of high-quality plasmid DNA
  • Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization

Spécifications

Content And Storage • Resuspension buffer, store at 4°C upon reciept
• Lysis buffer, store at room temperature
• Precipitation buffer, store at room temperature
• Binding buffer, store at room temperature
• Wash buffer 1, store at room temperature
• Wash buffer 2 (concentrate), store at room temperature
• Elution buffer, store at room temperature
• Column assemblies, store at room temperature
• Syringe filters, store at room temperature
• Syringe plungers, store at room temperature
• Collection tubes, store at room temperature
Format Kit
Isolation Technology Silica Column
Scale Midi
For Use With (Application) Cloning, PCR
No. of Reactions 50 Preps
Prep Scale Midi scale input culture of 25-100 mL to obtain yields of up to 200 μg
Product Line PureLink
Product Type Plasmid Purification Kit
Quantity 50 Preps
Shipping Condition Room Temperature
Test Time 30 min.
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For Research Use Only. Not for use in diagnostic procedures.