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Invitrogen™ PureLink™ Expi Endotoxin-Free Mega Plasmid Purification Kit
Description
The PureLink™ Expi Endotoxin-Free Mega Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange resin. Purify up to 5 mg of high quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines. The kit utilizes a novel vacuum-assisted purification workflow that dramatically reduces purification time to approximately 2 hours.
- Simple and fast protocol—unlike other anion exchange-based endotoxin-free plasmid purification systems, a vacuum filtration method is used for lysate clarification and plasmid DNA purification. There is no waiting for gravity-flow columns, and purification typically takes 2 hours.
- High yield—isolate up to 5 mg of high quality, endotoxin-free plasmid DNA
- Endotoxin-free plasmid—produces endotoxin-free (less than 0.1 EU/μg) plasmid that is ideal for sensitive applications such as transfection of primary cells and research on gene therapy or plasmid vaccines
- Purity—ultralow levels of protein, RNA, and genomic DNA contamination
- Compatibility—purified plasmid is compatible with the Gibco™ ExpiCHO™ and Expi293™ transient mammalian expression systems
- Endotoxin-free components—includes additional endotoxin-free elution buffer, water, and plasmid storage tubes
Specifications
Specifications
| Content And Storage | • Resuspension Buffer |
| Format | Spin Column |
| Isolation Technology | Anion Exchange Resin |
| Sample Type | Bacterial Culture |
| Expression System | Mammalian |
| For Use With (Application) | Cloning, PCR |
| High-throughput Compatibility | Manual Protocols |
| Prep Scale | Large Volume Microbial Samples (> 10 mL) |
| Product Line | PureLink |
| Product Type | Plasmid Purification Kit |
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Frequently Asked Questions (FAQs)
The anion exchange resin used in PureLink HiPure plasmid purification kits produce plasmid DNA with inherently low endotoxin levels of 0.1-1.0 EU/µg. To add to this, PureLink Expi Endotoxin-Free Mega/Giga plasmid purification kits contain endotoxin-free components, and a proprietary Endotoxin Removal Buffer that further enhances endotoxin depletion, resulting in plasmid DNA that is consistently endotoxin-free (< 0.1 EU/µg) .
No. Endotoxin Removal Buffer (ER) in PureLink Expi Endotoxin-Free Mega/Giga plasmid purification kits is not sold separately.
The PureLink Expi Endotoxin-Free Mega/Giga plasmid purification kits can produce endotoxin-free (< 0.1 EU/µg) advanced transfection-grade plasmid DNA.
For research use only. Not for use in diagnostic procedures.