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Invitrogen™ PureLink™ Expi Endotoxin-Free Giga Plasmid Purification Kit
Description
Includes
- Resuspension Buffer
- Lysis Indicator
- RNase A
- Lysis Buffer
- Precipitation Buffer
- Endotoxin Removal Buffer
- Wash Buffer
- Elution Buffer
- TE Buffer
- Expi Giga Filtration Column
- Expi Giga DNA Binding Column
- Endotoxin-free components: elution buffer, water, plasmid storage tubes
The PureLink™ Expi Endotoxin-Free Giga Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange resin. Purify up to 15 mg of high quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines. The kit utilizes a novel vacuum-assisted purification workflow that dramatically reduces purification time to approximately 2 hours.
- Simple and fast protocol—unlike other anion exchange-based endotoxin-free plasmid purification systems, a vacuum filtration method is used for lysate clarification and plasmid DNA purification. There is no waiting for gravity-flow columns, and purification typically takes 2 hours.
- High yield—isolate up to 15 mg of high quality, endotoxin-free plasmid DNA
- Endotoxin-free plasmid—produces endotoxin-free (less than 0.1 EU/μg) plasmid that is ideal for sensitive applications such as transfection of primary cells and research on gene therapy or plasmid vaccines
- Purity—ultralow levels of protein, RNA, and genomic DNA contamination
- Compatibility—purified plasmid is compatible with the Gibco™ ExpiCHO™ and Expi293™ transient mammalian expression systems
- Endotoxin-free components—includes additional endotoxin-free elution buffer, water, and plasmid storage tubes for added peace of mind
Specifications
Specifications
| Content And Storage | • Resuspension Buffer |
| Format | Spin Column |
| Isolation Technology | Anion Exchange Resin |
| Sample Type | Bacterial Culture |
| Expression System | Mammalian |
| For Use With (Application) | Cloning, PCR |
| High-throughput Compatibility | Manual Protocols |
| No. of Reactions | 2 Preps |
| Prep Scale | Large Volume Microbial Samples (> 10 mL) |
| Product Line | PureLink |
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Frequently Asked Questions (FAQs)
The anion exchange resin used in PureLink HiPure plasmid purification kits produce plasmid DNA with inherently low endotoxin levels of 0.1-1.0 EU/µg. To add to this, PureLink Expi Endotoxin-Free Mega/Giga plasmid purification kits contain endotoxin-free components, and a proprietary Endotoxin Removal Buffer that further enhances endotoxin depletion, resulting in plasmid DNA that is consistently endotoxin-free (< 0.1 EU/µg) .
No. Endotoxin Removal Buffer (ER) in PureLink Expi Endotoxin-Free Mega/Giga plasmid purification kits is not sold separately.
The PureLink Expi Endotoxin-Free Mega/Giga plasmid purification kits can produce endotoxin-free (< 0.1 EU/µg) advanced transfection-grade plasmid DNA.
For research use only. Not for use in diagnostic procedures.