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Invitrogenā„¢ PureLinkā„¢ Expi Endotoxin-Free Buffer Set DFS Item

Specifically, the buffer set includes RNAse A, Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), Equilibration Buffer (EQ1), Wash Buffer (W8), Elution buffer (E4), TE Buffer (TE), Endotoxin Removal Buffer, Lysis Indicator, and endotoxin free water.

Supplier:  Invitrogenā„¢ A33074

Catalog No. A33074


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Description

Description

Specifically, the buffer set includes RNAse A, Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), Equilibration Buffer (EQ1), Wash Buffer (W8), Elution buffer (E4), TE Buffer (TE), Endotoxin Removal Buffer, Lysis Indicator, and endotoxin free water.

  • Simple and super-fast protocolā€”Unlike other anion-exchange-based endotoxin free plasmid purification systems, the PureLinkā„¢ Expi Endotoxin-Free Maxi Plasmid Purification Kit utilizes vacuum filtration for lysate clarification and plasmid DNA purification. There is no waiting for gravity-flow columns and typical purification takes 90 minutes.
  • High yieldā€”solate up to 1.5 mg of high quality, endotoxin-free plasmid DNA
  • Endotoxin-Freeā€”Purify endotoxin-free (<0.1 EU/ Ī¼g) plasmid that is ideal for sensitive applications such as transfection of primary cells, genome engineering, and research on gene therapy or plasmid vaccines.
  • Purityā€”Ultralow levels of protein, RNA and genomic DNA contamination
  • Compatibility with ExpiCHOā„¢ and Expi293ā„¢ Expression Systemsā€”Plasmid purified using the PureLinkā„¢ Expi Endotoxin-Free Plasmid Purification Kit is compatible with the Gibcoā„¢ ExpiCHOā„¢ and Expi293ā„¢ transient mammalian expression systems.
Greener Choice Claims
  • Environmental benefits include:
    • Renewable Energy
    • Recyclable
    • Non-Toxic
Specifications

Specifications

ā€¢ Resuspension buffer
ā€¢ Lysis indicator
ā€¢ RNase A
ā€¢ Lysis buffer
ā€¢ Precipitation buffer
ā€¢ Endotoxin removal buffer
ā€¢ Wash buffer
ā€¢ Elution buffer
ā€¢ TE buffer
Bacteria
Cloning, PCR
> 1 mg (Large-Scale) Plasmid DNA, > 200 Ī¼g (Large-Scale) Plasmid DNA
Buffer Set
Room Temperature
90 min.
Maxi
Mammalian
Manual Protocols
PureLinkā„¢
1 Set
Plasmid DNA
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For Research Use Only. Not for use in diagnostic procedures.