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  6. MilliporeSigma™ Proteinase K Solution, 600mAU/mL

MilliporeSigma™ Proteinase K Solution, 600mAU/mL

Catalog No. 710494
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10 mL
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710494 10 mL
710493 2 mL
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Catalog No. 710494 Supplier MilliporeSigma™ Supplier No. 710494
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Description

Efficient removal of proteins from nucleic acid solutions

  • Proteinase K is a highly active 28,904-Da serine protease isolated from the fungus Tritirachium album 
  • The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA
  • Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C)
  • The recommended working concentration is 50-100μg/mL for protein removal and enzyme inactivation, and up to 2mg/mL for tissue treatment
  • The Proteinase K, Lyophilized powder can be prepared as a 20mg/mL stock solution in water and stored in aliquots at -20°C
  • The enzyme is also available as a ready-to-use concentrated stock solution (600mAU/mL) that is convenient for routine use in most applications
  • 1 mg of Proteinase K is the equivalent of 30mAU (AU = Anson unit)
  • The Novagen Proteinase K products are free of detectable DNase and RNase

Specifications

Content And Storage -20°C
Quantity 10 mL
Product Type Proteinase K