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  6. Thermo Scientific™ ProSwift™ RP-4H HPLC Columns

Thermo Scientific™ ProSwift™ RP-4H HPLC Columns
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Catalog No. DX069477
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50 mm
250 mm
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Catalog No. Length (Metric)
DX069477 50 mm
DX066640 250 mm
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Catalog No. DX069477 Supplier Thermo Scientific™ Supplier No. 069477
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Description

Run fast protein separations at high linear velocities with Thermo Scientific™ ProSwift™ RP-4H LC Columns.

Run fast protein separations at high linear velocities with Thermo Scientific™ ProSwift™ RP-4H LC Columns. These reversed-phase monolith columns deliver the outstanding resolving power of nonporous analytical media combined with faster separations and analysis than other bead-based columns. ProSwift polymer monolith columns in PEEK™ tubing achieve long column lifetime, excellent reproducibility even after hundreds of runs and have broad pH stability, providing high stringent wash capability (1M NaOH).

Each monolith is a single cylindrical polymer rod containing an uninterrupted, interconnected network of flow-through channels of a specific pore size. These large channels and the monolith's nonporous surfaces provide fast mass transfer for the high-resolution and fast protein separations. The monolith morphology results in low operating backpressure, allowing the use of higher linear velocities with minimal loss of resolution.

Advantages of High Resolution at Exceptionally High Flow Rates

  • Highest operational flow rates available
  • High throughput and improved productivity
  • Excellent stability over a wide pH range of 1 to 14
  • Outstanding reproducibility and ruggedness
  • High loading capacity
Fast, Sensitive Protein Separations
  • High speed protein separations
  • High sensitivity for LC-MS
  • High column-to-column reproducibility
  • Wide range of column I.D. and lengths available
  • Superior lifetime
Easy Column Installation
  • Use Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fittings
Preconcentrate and Desalt Samples   
  • Precolumns are available to preconcentrate and desalt samples.
  • You can also use Thermo Scientific™ PepSwift™ Trap Columns for preconcentration and desalting of samples consisting of peptides and proteins without negatively impacting the chromatographic performance or recovery of the compounds.
  • Change the selectivity of the separation or improve the trapping efficiency by employing various ion-pairing agents in the loading solvent and/or mobile phases.

Specifications

Column Chemistry Phenyl
Column Format Analytical Column
Diameter (Metric) 1 mm
Flow Rate 0.1 to 0.4 mL/min
For Use With LC/MS
Length (Metric) 50 mm
Max. Pressure 2000 psi (140 bar)
Particle Size Monolith
pH 1 to 14
Pore Size Monolith
Quantity Each
Solvents Most common Organic Solvents
Temperature 70°C
Stationary Phase Phenyl (Ph)
Product Line Monolith
Packing Material PSDVB (Polystyrene Divinylbenzene)
Column Type Reversed Phase
Phase Reversed Phase
Type Analytical Column
Technique Bio-LC
Green Features FSC
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Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.