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Promega™ Viral ToxGlo™ Assay Systems

Determines viral-induced cytopathic effects (CPE) in host cells caused by lytic virions

$148.73 - $683.73

Specifications

Detection Method Luminescence
Product Type Assay Systems
For Use With (Application) Determining viral-induced cytopathic effects (CPE) in host cells caused by lytic virions
Storage Requirements -30°C to -10°C.
View More Specs

 Alert

This method is only useful for viruses that produce cytotoxicity and CPE. Not for use in diagnostic procedures.

 Disclaimers

Research use only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

PRG8943

 
promega™
G8943
1 x 100mL Each for $535.00

PRG8942

 
promega™
G8942
10 x 10mL Each for $683.73

PRG8941

 
promega™
G8941
1 x 10mL Each for $148.73
Description & Specifications

Specifications

Detection Method Luminescence
Product Type Assay Systems
For Use With (Application) Determining viral-induced cytopathic effects (CPE) in host cells caused by lytic virions
Storage Requirements -30°C to -10°C.

The assay measures cellular ATP as a surrogate measure of host cell viability. When CPE occurs because of viral infection, ATP depletion can be measured and correlated with viral burden. The amount of ATP detected is directly proportional to the number of viable host cells in culture and can be used as a simple method to quantify viral-induced CPE.

  • Provide quantifiable data by luminescence detection, which obviates subjective operator error associated with visual scoring methods
  • Data can be recorded and analysis begun 10 minutes after reagent addition
  • Homogeneous “add-mix-measure” protocol reduces manual steps required for CPE assessment
  • “Glow-type” luminescent signal is generated that is proportional to the amount of ATP present
  • Cell washing, multiple pipetting steps and visual assessment are not required to assess CPE
  • System detects as few as 15 cells/well in a 384-well format in 10 minutes after reagent addition and mixing
  • Reagent is scalable from 96- to 1536-well plate formats (multiwell formats), making it ideal for automated high-throughput screening (HTS)
  • Luminescent signal is very stable with half-life generally >5 hours dependent on cell type and medium used, allowing batch or consecutive processing
  • No fluorescence interference results in high signal to background and delivers excellent Z values in screening applications

  • Determine viral infectivity and corresponding tissue culture infective dose
  • Determine potential antiviral potency or off-target toxicity of test compounds