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Promega™ Trypsin Gold, Mass Spectrometry Grade DFS Item

Manufactured to provide maximum specificity

Manufacturer:  Promega™ V5280

Catalog No. PR-V5280



Description

Description

Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. To produce Trypsin Gold, Mass Spectrometry Grade, lysine residues in the porcine trypsin are modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion. The specificity of the purified trypsin is further improved by TPCK treatment, which inactivates chymotrypsin. The treated trypsin is then purified by affinity chromatography and lyophilized to yield Trypsin Gold, Mass Spectrometry Grade.

  • Each lot is qualified by mass spectrometry
  • Stability ensured up to five freeze-thaw cycles, minimizing leftover reagents

Specifications

Specifications

Trypsin Gold
White
7.7
Trypsin digestion of Proteins from 1 or 2-D Gel, tryptic digestion of Proteins in-solution
−30 to −10°C
Lyophilized Powder
Antipain (50μg/mL), Antithrombin (1U/mL), APMSF (0.01 to 0.04mg/mL), Aprotinin (0.06 to 2μg/mL), Leupeptin (0.5μg/mL), PMSF (17 to 170μg/mL), TLCK (37 to 50μg/mL)
100μg
50mM Acetic Acid Resuspension Buffer
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