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Promega T7 RNA Polymerase 
DNA-dependent RNA polymerase for in vitro transcription.
Supplier: Promega P2077
Description
- DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences
- >90% pure as determined by SDS polyacrylamide gel electrophoresis
- Free of detectable levels of contaminating RNase and DNase activity (
- Incorporates 32P, 33P, 3H and 35S nucleoside triphosphates
Specifications
| 10 to 20 U/μL | |
| 5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl | |
| 7.7 | |
| 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol | |
| Recombinant E. coli strain | |
| T7 RNA Polymerase | |
| Liquid |
| Colorless | |
| >90% | |
| Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA | |
| -30°C to -10°C | |
| 5000 U | |
| Recombinant RNasin™ Ribonuclease |