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Promega T7 RNA Polymerase

Catalog No. PRP2077
Encompass
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Quantity:
1000 U
5000 U
Concentration:
10 to 20 U/μL
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Catalog No. Quantity Concentration
PRP2077 5000 U 10 to 20 U/μL
PRP2075 1000 U 10 to 20 U/μL
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Catalog No. PRP2077 Supplier Promega Supplier No. P2077

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

DNA-dependent RNA polymerase for in vitro transcription.

  • DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences
  • >90% pure as determined by SDS polyacrylamide gel electrophoresis
  • Free of detectable levels of contaminating RNase and DNase activity (
  • Incorporates 32P, 33P, 3H and 35S nucleoside triphosphates

Specifications

Concentration 10 to 20 U/μL
Color Colorless
Components 5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
Purity >90%
pH 7.7
For Use With (Application) Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Storage Buffer 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Content And Storage -30°C to -10°C
Source Recombinant E. coli strain
Quantity 5000 U
Product Type T7 RNA Polymerase
Inhibitors Recombinant RNasin™ Ribonuclease
Form Liquid
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