missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Modifying Enzymes
  5. PRM1051

Promega T4 RNA Ligase

Catalog No. PRM1051
Encompass
Change view
Click to view available options
Quantity:
500 U
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PRM1051 500 U
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PRM1051 Supplier Promega Supplier No. M1051

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA.

  • Joins ssRNA or ssDNA onto the 5′-Phosphoryl End of ssRNA or ssDNA
  • Adds [5′-32P] nucleoside 3′,5′-bis (phosphate) onto single-stranded RNA
  • Can be inactivated by heating at 65°C for 15 minutes
  • Supplied at a concentration of 10u/μL

Specifications

Concentration 10 U/μL
Color Colorless
Components 10X Reaction Buffer: 500mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 50mM DTT, 10mM ATP
Purity >90%
pH 7.5
For Use With (Application) Ligation of single-stranded Nucleic Acids, 3' end-labeling of RNA, circularization of RNA and DNA Oligonucleotides, cloning full length cDNA
Storage Buffer 10mM Tris (pH 7.5 at 25°C), 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.1% Tween™20, 50% Glycerol
Content And Storage -30°C to -10°C
Source Recombinant E. coli strain
Quantity 500 U
Product Type T4 RNA Ligase
Molecular Weight (g/mol) 43.5
Form Liquid
Show More Show Less