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Promega T4 Polynucleotide Kinase Buffer Pack

Catalog No. PRC1313
Encompass
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3 x 500 μL
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Catalog No. Quantity
PRC1313 3 x 500 μL
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Catalog No. PRC1313 Supplier Promega Supplier No. C1313

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Description

T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group.

  • Transfers the γ-Phosphate from ATP to the 5′ End of Nucleotides
  • Can be inactivated by heating at 68°C for 10 minutes
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Labels the 5′ end of ssDNA, dsDNA and RNA molecules for use as probes or for sequencing or DNA-protein footprinting
  • Supplied at a concentration of 5-10u/μL

Specifications

Color Colorless
Components 3 Tubes of T4 DNA Kinase 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25degC), 100mM MgCl2, 50mM DTT
pH 8
For Use With (Application) For T4 DNA Kinase Enzyme
Content And Storage -30°C to -10°C
Quantity 3 x 500 μL
Product Type 4 DNA Kinase Buffer Pack
Form Liquid