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Promega™ T4 Polynucleotide Kinase DFS Item

Catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. Promega T4 Polynucleotide Kinase Enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.

$63.00 - $457.00

Specifications

Color Colorless
Product Name T4 Polynucleotide Kinase
For Use With (Application) 5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Includes 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
Inhibitors Ammonium Ions and low levels of Phosphate Buffers
View More Specs


Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
PR-M4101 Promega™
M4101
100U Each for $63.00
PR-M4103 Promega™
M4103
1000U Each for $457.00
Specifications

Description

  • May Be Heat-Inactivated: T4 Polynucleotide Kinase may be inactivated by heating at 68°C for 10 minutes
  • Provided with 10X Reaction Buffer: 700mM Tris-HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
  • Blue/White Cloning Qualified: Promega's blue/white cloning assay provides higher level of quality control for enzymes used in cloning applications
Applications:
  • 5´ end-labeling of single- or double-stranded DNA and RNA molecules for use as probes, for sequencing or for DNA-protein footprinting
  • Phosphorylation of DNA prior to cloning
Description & Specifications

Specifications

Colorless
5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Ammonium Ions and low levels of Phosphate Buffers
7.5
Recombinant E. coli strain
-20°C
T4 Polynucleotide Kinase
10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
132
≥90%
20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol