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Promega T4 Polynucleotide Kinase 
T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group.
$77.08 - $567.16
Specifications
| Concentration | 5 to 10 U/μL |
|---|---|
| Color | Colorless |
| Components | 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT |
| Purity | ≥90% |
| pH | 7.5 |
Description
- Transfers the γ-Phosphate from ATP to the 5′ End of Nucleotides
- Can be inactivated by heating at 68°C for 10 minutes
- Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
- Labels the 5′ end of ssDNA, dsDNA and RNA molecules for use as probes or for sequencing or DNA-protein footprinting
- Supplied at a concentration of 5-10u/μL
Specifications
| 5 to 10 U/μL | |
| 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT | |
| 7.5 | |
| 20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol | |
| Recombinant E. coli strain | |
| 132 | |
| Liquid |
| Colorless | |
| ≥90% | |
| 5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning | |
| -30°C to -10°C | |
| T4 Polynucleotide Kinase | |
| Ammonium Ions and low levels of Phosphate Buffers |