Please login to your online account to display your discounted pricing

Promega™ T4 Polynucleotide Kinase

Catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. Promega T4 Polynucleotide Kinase Enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.

$63.00 - $457.00

Specifications

Color Colorless
Product Name T4 Polynucleotide Kinase
For Use With (Application) 5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Includes 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
Inhibitors Ammonium Ions and low levels of Phosphate Buffers
View More Specs
Products
Catalog Number Mfr. No. Quantity Price Quantity    

PR-M4101

 
promega™
M4101
100U Each for $63.00

PR-M4103

 
promega™
M4103
1000U Each for $457.00
Description & Specifications

Specifications

Color Colorless
Product Name T4 Polynucleotide Kinase
For Use With (Application) 5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Includes 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
Inhibitors Ammonium Ions and low levels of Phosphate Buffers
Molecular Weight 132
pH 7.5
Purity ≥90%
Source Recombinant E. coli strain
Storage Buffer 20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Storage Requirements -20°C

  • May Be Heat-Inactivated: T4 Polynucleotide Kinase may be inactivated by heating at 68°C for 10 minutes
  • Provided with 10X Reaction Buffer: 700mM Tris-HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
  • Blue/White Cloning Qualified: Promega's blue/white cloning assay provides higher level of quality control for enzymes used in cloning applications
Applications:
  • 5´ end-labeling of single- or double-stranded DNA and RNA molecules for use as probes, for sequencing or for DNA-protein footprinting
  • Phosphorylation of DNA prior to cloning