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Promega T4 Polynucleotide Kinase

Catalog No. PRM4103
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1000 U
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PRM4103 1000 U
PRM4101 100 U
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Catalog No. PRM4103 Supplier Promega Supplier No. M4103

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Description

T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group.

  • Transfers the γ-Phosphate from ATP to the 5′ End of Nucleotides
  • Can be inactivated by heating at 68°C for 10 minutes
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Labels the 5′ end of ssDNA, dsDNA and RNA molecules for use as probes or for sequencing or DNA-protein footprinting
  • Supplied at a concentration of 5-10u/μL

Specifications

Concentration 5 to 10 U/μL
Color Colorless
Components 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
Purity ≥90%
pH 7.5
For Use With (Application) 5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Storage Buffer 20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Content And Storage -30°C to -10°C
Source Recombinant E. coli strain
Quantity 1000 U
Product Type T4 Polynucleotide Kinase
Molecular Weight (g/mol) 132
Inhibitors Ammonium Ions and low levels of Phosphate Buffers
Form Liquid
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