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Promega T4 DNA Polymerase 
High-fidelity polymerase useful for mutagenesis reactions, 3'-overhang removal and 5'-overhang fill-in reactions.
Supplier: Promega M4215
Description
- Synthesizes DNA 5′→3′ from a Primed ssDNA Template
- High fidelity enzyme of choice for applications where misincorporation is a concern
- Flexible polymerase used in a variety of molecular applications; active in many Promega 1X restriction enzyme buffers
- Heat inactivated by heating at 75°C for 10 minutes
- Supplied at a concentration of 5-10u/μL
Specifications
| 5 to 10 U/μL | |
| 10X Reaction Buffer: 250mM Tris acetate (pH 7.7), 1M Potassium Acetate, 100mM Magnesium Acetate and 10mM DTT | |
| 6.5 | |
| 200mM Potassium Phosphate (pH 6.5 at 25°C), 2mM DTT, 50% Glycerol | |
| Recombinant E. coli strain | |
| T4 DNA Polymerase | |
| Liquid |
| Colorless | |
| >95% | |
| Flush 5' protruding ends with labeled or unlabeled dNTPs, blunt 3' overhangs, in-vitro mutagenesis | |
| -30°C to -10°C | |
| 500 U | |
| 112 |