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Promega™ T4 DNA Ligase

Enzymes are available in convenient, cost-effective concentrations that are economical and easy to pipet

$54.00 - $221.00

Specifications

Color Colorless
Product Name T4 DNA Ligase
For Use With (Application) Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Includes 10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP
Molecular Weight 68
View More Specs
Products
Catalog Number Mfr. No. Concentration Quantity Price Quantity    

PR-M1801

 
promega™
M1801
1 to 3U/μL 100U Each for $54.00

PR-M1794

 
promega™
M1794
10 to 20U/μL 500U Each for $221.00

PR-M1804

 
promega™
M1804
1 to 3U/μL 500U Each for $216.00
Description & Specifications

Specifications

Color Colorless
Product Name T4 DNA Ligase
For Use With (Application) Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Includes 10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP
Molecular Weight 68
pH 7.4
Purity >90%
Source Recombinant E. coli strain
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Storage Requirements -20°C

T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration. The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule but will not join single-stranded nucleic acids.