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  5. Promega T4 DNA Ligase

Promega T4 DNA Ligase

Catalog No. p-3509032
Encompass
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Quantity:
100 U
500 U
Concentration:
1 to 3 U/μL
10 to 20 U/μL
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Catalog No. Quantity Concentration
PRM1801 100 U 1 to 3 U/μL
PRM1794 500 U 10 to 20 U/μL
PRM1804 500 U 1 to 3 U/μL
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Catalog No. PRM1801 Supplier Promega Supplier No. M1801
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Description

For ligation of DNA ends.

  • Joins Two DNA Strands with Cohesive or Blunt Ends
  • Use with 5′, 3′ or blunt-ended dsDNA (e.g., inserts and vectors)
  • Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications
  • Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule

Specifications

Concentration 1 to 3 U/μL
Color Colorless
Components 10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP
Purity >90%
pH 7.4
For Use With (Application) Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Content And Storage -30°C to -10°C
Source Recombinant E. coli strain
Quantity 100 U
Product Type T4 DNA Ligase
Molecular Weight (g/mol) 68
Form Liquid
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