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Promega T3 RNA Polymerase 
DNA-dependent RNA polymerase for in vitro transcription.
Supplier: Promega P4024
Description
- DNA-Dependent RNA Polymerase Specific for T3 Promoter Sequences
- Exhibits extremely high affinity and specificity for T3 promoter sequences
- >90% pure as determined by SDS polyacrylamide gel electrophoresis
- Free of detectable levels of contaminating RNase and DNase activity (
- Supplied at a concentration of 10-20u/μL (Cat.# P2083) and 80u/μL (Cat.# P4024)
Specifications
| 8 U/μL | |
| 100mM DTT 5X Reaction Buffer, transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl | |
| 7.7 | |
| 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol | |
| Recombinant E. coli strain | |
| T3 RNA Polymerase |
| Colorless | |
| >90% | |
| Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA | |
| -30°C to -10°C | |
| 2500 U | |
| Liquid |