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Promega pRL Family of Renilla Luciferase Control Reporter Vectors DFS Item

The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used in combination with a firefly luciferase vector to cotransfect mammalian cells.

Supplier:  Promega E2231

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Numéro de catalogue. PR-E2231


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Description

Description

  • Four Vectors that Contain Wild-Type Renilla Luciferase for Normalization in Reporter Assays
  • A T7 promoter is located immediately upstream of Rluc for in vitro synthesis of Renilla luciferase
  • The SV40 late poly(A) signal sequence is positioned downstream of Rluc to provide efficient transcription termination and mRNA polyadenylation
  • A prokaryotic origin of replication and β-lactamase gene allow selected propagation of the pRL vectors in E. coli host strains
Spécifications

Spécifications

SV40
TE Buffer (pH 7.4)
20 μg
Provide constitutive expression of Renilla reniformis (sea pansy) Luciferase, can be used in combination with a firefly Luciferase vector to co-transfect mammalian cells
Luciferase
-30°C to -10°C
pRL-SV40 vector
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Licensed from University of Georgia Research Foundation, Inc., under U.S. Patent 5,418,155; Canadian Patent 2,105,984 and related patents.