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Promega™ pRL Family of Renilla Luciferase Control Reporter Vectors

May be used in combination with a firefly luciferase vector to cotransfect mammalian cells

$386.27 - $387.34

Specifications

For Use With (Application) Provide constitutive expression of Renilla reniformis (sea pansy) Luciferase, can be used in combination with a firefly Luciferase vector to co-transfect mammalian cells
Quantity 20μg
Reporter Gene Luciferase
Storage Buffer TE Buffer (pH 7.4)
Storage Requirements -20°C
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 Disclaimers

Licensed from University of Georgia Research Foundation, Inc., under U.S. Patent 5,418,155; Canadian Patent 2,105,984 and related patents.

Products
Catalog Number Mfr. No. Vector Price Quantity    

PR-E2271

 
promega™
E2271
pRL-null vector Each for $386.27

PR-E2241

 
promega™
E2241
pRL-TK vector Each for $387.34

PR-E2261

 
promega™
E2261
pRL-CMV vector Each for $387.34

PR-E2231

 
promega™
E2231
pRL-SV40 vector Each for $387.34
Description & Specifications

Specifications

For Use With (Application) Provide constitutive expression of Renilla reniformis (sea pansy) Luciferase, can be used in combination with a firefly Luciferase vector to co-transfect mammalian cells
Quantity 20μg
Reporter Gene Luciferase
Storage Buffer TE Buffer (pH 7.4)
Storage Requirements -20°C

  • These vectors provide constitutive expression of Renilla reniformis (sea pansy) luciferase
  • Expression of Renilla luciferase provides an internal control value to which expression of the experimental firefly luciferase reporter gene may be normalized
  • A T7 promoter is located immediately upstream of Rluc, allowing in vitro synthesis of Renilla luciferase
  • The SV40 late poly(A) signal sequence is positioned downstream of Rluc to provide efficient transcription termination and mRNA polyadenylation
  • In addition to the modified Rluc reporter gene, all pRL Vectors are isolated from a dam–/dcmE. coli K host strain, allowing digestion with restriction enzymes that are sensitive to dam and dcm methylation
  • A prokaryotic origin of replication and β-lactamase gene allow selected propagation of the pRL vectors in E. coli host strains

  • Four different promoter configurations are available
  • The HSV-thymidine kinase promoter (pRL-TK) is relatively weak and may be particularly useful in providing neutral constitutive expression of the Renilla luciferase control reporter
  • The early SV40 enhancer/promoter region (pRL-SV40) and the CMV immediate early enhancer/promoter region (pRL-CMV) typically provide high-level transcription and, therefore, may be less suitable for coreporter applications involving experimental vectors with robust regulatory elements