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Promega™ ONE-Glo™ Luciferase Assay System

Provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells

$188.32 - $1,209.10

Specifications

Detection Method Luminescence
For Use With (Application) Detection of firefly luciferase reporter gene expression in mammalian cells
For Use With (Equipment) 384- and 1536-well plates
pH 6.7
Storage Requirements -20°C
View More Specs
Includes:  ONE-Glo Luciferase Assay Buffer, ONE-Glo Luciferase Assay Substrate

 Disclaimers

Patent pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673.
Certain applications of this product may require licenses from others.

Products
Catalog Number Mfr. No. Includes Quantity Price Quantity    

PRE6110

 
promega™
E6110
1 x 10mL ONE-Glo™ Luciferase Assay Buffer, 1 x 1 vial ONE-Glo™ Luciferase assay substrate 10mL Each for $188.32

PRE6120

 
promega™
E6120
1 x 100mL ONE-Glo™ Luciferase Assay Buffer, 1 x 1 vial ONE-Glo™ Luciferase assay substrate 100mL Each for $1,209.10
Description & Specifications

Specifications

Detection Method Luminescence
For Use With (Application) Detection of firefly luciferase reporter gene expression in mammalian cells
For Use With (Equipment) 384- and 1536-well plates
pH 6.7
Storage Requirements -20°C

  • Utilizes a novel luciferase substrate (5'-fluoroluciferin) that is less sensitive to culture media, phenol red, and luciferase inhibitors than other luciferase reagents
  • Generates an extended, bright light output that allows high sensitivity, especially when extended incubation is required prior to reading results
  • Less sensitive to mixing and dispensing conditions, enhancing reproducibility and making the assay ideal for use in 384- and 1536-well microplates
  • Offers extended stability at room temperature and 4°C for more convenient everyday use