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Promega M-MLV Reverse Transcriptase, RNase H Minus

Catalog No. PRM5301
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Quantity:
10,000 U
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Catalog No. Quantity
PRM5301 10,000 U
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Catalog No. PRM5301 Supplier Promega Supplier No. M5301

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Deletion mutation of M-mLV RT resulting in no RNase H activity. Suitable for rare or long transcripts.

  • Helpful for Preparing Long cDNAs and Libraries with a High Percentage of Full-Length cDNA
  • Supplied at a concentration of 100-200u/μL

Specifications

Concentration 100 to 200 U/μL
Color Colorless
Components 5X Reaction Buffer: 250mM Tris HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT
Purity >90%
pH 7.5
For Use With (Application) First-strand cDNA synthesis, primer extension
Storage Buffer 20mM Tris HCl (pH 7.5 at 25°C), 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet™ P-40, 50% Glycerol
Content And Storage -30°C to -10°C
Source Recombinant E. coli strain
Quantity 10,000 U
Product Type M-MLV Reverse Transcriptase, RNase (H-)
Inhibitors Recombinant RNasin™ Ribonuclease
Form Liquid
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