Promega™ GoTaq™ qPCR Master Mix 

Description

The system contains a new proprietary fluorescent DNA binding dye, BRYT™ GREEN, that exhibits greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR™ Green I. Thermal activation is achieved by incubating the assembled reaction at 95°C for two minutes. The proprietary polymerase/buffer formulation accommodates extended cycle numbers (45–50 cycles) and is compatible with thermal cycling programs that require extended activation (95°C for 10 minutes).

• Pre-mixed, ready-to-use stabilized 2X formulation that includes all components for qPCR except sample DNA, primers and water to dilute the DNA standards
• Detection of low copy targets
• Enhanced stability for automated setup
• Direct substitute for SYBR™ Green I products
• The robust, reliable performance of GoTaq™ Hot Start Polymerase
• The Promega PCR Performance Guarantee

Features - Benefits

Brighter Signal: Sensitive detection for earlier quantitation of low- and high-copy targets.
Enhanced Stability: Exceptional room-temperature setup makes it suitable for automation and high-throughput detection.
Versatile: Compatible with both fast and standard qPCR cycling methods.
Robust: High-efficiency, full-length cDNA synthesis in the presence of inhibitors.
Confidence: The Promega PCR Performance Guarantee.

These catalog numbers are not available in Europe. Use of these products in all other countries except the U.S. is covered by one or more of the following U.S. patents and corresponding patent claims outside the U.S.: 5,079,352; 5,789,224; 5,618,711; 6,127,155 and claims outside the U.S. corresponding to U.S. Patent No. 4,889,818. The purchase of this product includes a limited, nontransferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404 USA. Use of these products in the U.S. for basic PCR is outside of any valid U.S. patents assigned to Hoffman La-Roche or Applera.
These products can be used in the U.S. for basic PCR in research, commercial or diagnostic applications without any license or royalty fees.

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