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Promega™ GloSensor™ cAMP Assay

Provides an extremely sensitive and easy-to-use format for the interrogation of overexpressed or endogenous G-protein coupled receptors (GPCRs) that signal via changes in the intracellular concentration of cAMP

$510.39 - $2,034.07


Detection Method Luminescence
Format Fluid
For Use With (Application) Full and partial agonists and antagonists as well as inverse agonists and allosteric modulators
View More Specs
Requires:  GloSensor cAMP Reagent is required for use with this assay per the GloSensor Limited Use Label License.


By use of this product, researcher agrees to be bound by the terms of this limited use statement. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Not for use in live animals—For use in live animals contact Promega regarding the availability of licenses. Researchers may use this product and its derivatives in their own research solely for the luminescent determination of cAMP levels in vitro or within cells (excluding uses in living animals). Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence encoding the GloSensor cAMP Reporter of this product (i.e., the bioluminescent chimeric protein comprising luciferase and cAMP-binding domains). Researchers may transfer derivatives to others provided that recipients agree to be bound by the conditions of this limited use statement prior to such transfer. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use the GloSensor cAMP Reagent purchased from Promega for all determinations of luminescence activity of this product and derivatives; or (2) contact Promega to obtain a license for use of the product and derivatives in conjunction with luminescent assay reagents not purchased from Promega. Promega makes no representations or warranties of any kind, either expressed to implied, including for merchantability or fitness for a particular purpose with regards to the product. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
Mfr. No. E2301, E1171: U.S. Patent 5,670,356.
Mfr. No. E2301, E1171: Australian Patent 2001 285278 and other patents pending.
Mfr. No. E1261: Commercial use of this cell line requires a license from AdVec Inc.
Mfr. No. E1261: HEK293 cells were obtained under license from AdVec Inc.
Mfr. Nos. E1261, E2301, E1171: The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

Catalog Number Mfr. No. Includes Quantity Storage Requirements Price Quantity    


2 vials of 2 × 106; GloSensor cAMP HEK293 Cell Line 2 Vials -140°C CALL N/A N/A
 Please call Customer Service at 1-800-234-7437 or send an email to for assistance.


1 x 20μg pGlo Sensor 20F cAMP plasmid 20μg -20°C Each for $838.88


1 x 250mg GloSensor cAMP Reagent 250mg -70°C Each for $2,034.07


1 x 25mg GloSensor cAMP Reagent 25mg -70°C Each for $510.39
Description & Specifications


Detection Method Luminescence
Format Fluid
For Use With (Application) Full and partial agonists and antagonists as well as inverse agonists and allosteric modulators

The assay uses genetically encoded biosensor variants with cAMP binding domains fused to mutant forms of Photinus pyralis luciferase. Upon binding to cAMP, conformational changes occur that promote large increases in light output. Following pre-equilibration with substrate, cells transiently or stably expressing a biosensor variant can be used to assay GPCR function using a live-cell, nonlytic assay format, enabling simple kinetic measurements of cAMP accumulation or turnover in living cells.

  • Broad dynamic range with up to 1000-fold changes in light output obtained
  • Easy monitoring of cAMP in live cells enables a more complete analysis of receptor biology
  • High sensitivity assay permits easy detection of low-abundance, endogenous receptors
  • Direct detection of Gi-coupled receptor activation and inverse agonist activity in the absence of artificial stimulation by compounds such as forskolin provides increased biological relevance
  • Live-cell, nonlytic assay format greatly facilitates HTS/uHTS

Choice of Two Biosensor Plasmids

  • pGloSensor™-22F cAMP Plasmid (Mfr. No. E2301) is the first choice for most applications
  • Following cell-free expression in vitro, the biosensor encoded by pGloSensor-22F cAMP Plasmid shows an increased EC50 for activation together with increased signal-to-background ratio at cAMP saturation relative to the version encoded by the pGloSensor-20F cAMP construct
  • In general, similar relationships have been observed between the two constructs when their performance is compared in living cells
  • pGloSensor-20F cAMP Plasmid (Mfr. No. E1171) encodes a version of the biosensor that performs well in HEK293 cells at 37°C Luminescence from the pGloSensor-22F cAMP Plasmid construct can be more difficult to detect at physiologic temperatures

Screen for full and partial agonists and antagonists as well as inverse agonists; Screen for allosteric modulators; Profile Gs- and Gi-coupled receptor biology; Characterize cAMP changes in live cells; Assay pharmacology of compounds in live cells