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Promega™ BL21(DE3)pLysS Competent Cells

Allow high-efficiency protein expression of genes under the control of a T7 promoter and having a ribosome binding site

$271.78 - $271.78

Specifications

For Use With (Application) Allow high-efficiency Protein expression of genes under the control of a T7 promoter and having a ribosome binding site
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Products
Catalog Number Mfr. No. Includes Quantity Price Quantity    

PRL1195

 
promega™
L1195
BL21(DE3)pLysS competent cells >10 x 6cfu/μg 20 x 50μL Each for $271.78
Description & Specifications

Specifications

For Use With (Application) Allow high-efficiency Protein expression of genes under the control of a T7 promoter and having a ribosome binding site

BL21(DE3)pLysS is lysogenic for λ-DE3 (1), which contains the T7 bacteriophage gene I, encoding T7 RNA polymerase (2) under the control of the lac UV5 promoter. BL21(DE3)pLysS also contains a plasmid, pLysS, which carries the gene encoding T7 lysozyme. T7 lysozyme lowers the background expression level of target genes under the control of the T7 promoter but does not interfere with the level of expression achieved following induction by IPTG.

  • Contains an IPTG-inducible gene for T7 RNA polymerase
  • Time-saving alternative to making competent cells
  • 1mL is sufficient for ten transformations
  • Available in 200μL aliquots
  • Also available for single-use in 50μL aliquots