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Promega Alkaline Phosphatase, Calf Intestinal
Description
- Catalyzes the Hydrolysis of 5′ Phosphate Groups from DNA, RNA, and r- and dNTPs
- Available at high concentration; Cat.# M2825 contains 1,000 units of CIAP at 20u/μL
- Qualified for blue/white cloning; our assay provides a higher level of quality control for enzymes used in cloning applications
Specifications
Specifications
| Concentration | 20 U/μL |
| Color | Colorless |
| Components | 10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine |
| pH | 8 |
| For Use With (Application) | Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase |
| Storage Buffer | 10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol |
| Content And Storage | -30°C to -10°C |
| Source | Calf intestinal mucosa |
| Quantity | 1000 U |
| Product Type | Alkaline Phosphatase |
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