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Invitrogen™ ProBond™ Nickel-Chelating Resin
Description
Includes
The ProBond resin is provided as a 50% slurry in 20% ethanol.
- ProBond™ Nickel-Chelating Resin is a nickel-charged affinity resin used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
- Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine
- One-step purification can be performed under both native and denaturing conditions
- ProBond™ Resin uses the chelating ligand iminodiacetic acid (IDA) coupled to a highly cross-linked 6% agarose resin
- Suitable for use in FPLC, batch, and gravity flow applications
- Guaranteed stable for 6 months when properly stored at 4°C
Specifications
Specifications
| Content And Storage | The ProBond™ resin is pre-charged and able to bind 1-5 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 20% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. ProBond™ resin is guaranteed stable for 6 months when properly stored. |
| Stationary Phase | Nickel-Chelating |
| Product Line | ProBond |
| Type | Resin |
| Quantity | 50 mL |
| Form | Suspension |
For Research Use Only. Not for use in diagnostic procedures.