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Invitrogen™ ProBond™ Nickel-Chelating Resin

Catalog No. R80101
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50 mL
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R80101 50 mL
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Catalog No. R80101 Supplier Invitrogen™ Supplier No. R80101
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Includes

The ProBond resin is provided as a 50% slurry in 20% ethanol.

A nickel-charged affinity resin used to purify recombinant proteins containing a polyhistidine (6xHis) sequence.

  • ProBond™ Nickel-Chelating Resin is a nickel-charged affinity resin used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
  • Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine
  • One-step purification can be performed under both native and denaturing conditions
  • ProBond™ Resin uses the chelating ligand iminodiacetic acid (IDA) coupled to a highly cross-linked 6% agarose resin
  • Suitable for use in FPLC, batch, and gravity flow applications
  • Guaranteed stable for 6 months when properly stored at 4°C

Specifications

Content And Storage The ProBond™ resin is pre-charged and able to bind 1-5 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 20% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. ProBond™ resin is guaranteed stable for 6 months when properly stored.
Stationary Phase Nickel-Chelating
Product Line ProBond
Type Resin
Quantity 50 mL
Form Suspension

For Research Use Only. Not for use in diagnostic procedures.