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Molecular Probes™ Probenecid, Water Soluble

Catalog No. P36400
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10 x 77 mg
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P36400 10 x 77 mg
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Catalog No. P36400 Supplier Molecular Probes™ Supplier No. P36400

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Probenecid, Water Soluble

Probenecid is commonly used to inhibit organic-anion transporters located in the cell membrane. Such transporters can extrude dyes and indicators and thus contribute to poor loading or a high background signal in assays based on retention of the dyes or indicators inside cells. The use of probenecid to block the efflux of intracellular dyes was first described by Di Virgilio et al. (1990), and it has been used with a wide range of anionic dyes and conjugates. The commonly used free acid form of probenecid is difficult to dissolve, requiring 1 M NaOH to get it into solution. Our water-soluble probenecid (P36400) dissolves quickly in assay buffer and eliminates the need to handle caustic NaOH. The water-soluble probenecid is included in the Fluo-4 NW Calcium Assay Kits (F36205, F36206) and is also available separately.

Specifications

Quantity 10 x 77 mg
Shipping Condition Room Temperature
Physical Form Solid
Product Type Cell Labeling Reagent

Frequently Asked Questions (FAQs)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).
I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

For Research Use Only. Not for use in diagnostic procedures.