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Applied Biosystems™ PrepSEQ™ Residual DNA Sample Preparation Kit
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Catalog No. 4413686
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100 preps
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4413686 100 preps
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Catalog No. 4413686 Supplier Applied Biosystems™ Supplier No. 4413686*
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Description

The Applied Biosystems PrepSEQ Residual DNA Sample Preparation Kit is optimized for highly efficient DNA recovery from complex mixtures of proteins, buffers, and salts which are typical of samples that need to be quantitated for residual host cell line DNA.

The Applied Biosystems PrepSEQ Residual DNA Sample Preparation Kit is optimized for highly efficient DNA recovery from complex mixtures of proteins, buffers, and salts which are typical of samples that need to be quantitated for residual host cell line DNA. Quantitative and consistent recovery of DNA can be obtained from challenging matrices, including samples that have high protein concentration, low pH, or high salt.

Specifications

Content And Storage Includes buffers, magnetic beads and enzymes for DNA extraction for residual DNA quantitation for 100 reactions
Isolation Technology Magnetic Bead
Scale Mini
Sample Type Biopharmaceutical Production Samples
Final Product Type Genomic DNA
For Use With (Application) Impurity Testing
High-throughput Compatibility Not High-throughput Compatible (Manual), Automated Protocols
No. of Reactions 100 preps
Product Line PrepSEQ
Quantity 100 preps
Sample Volume 100 μL
Target Genomic DNA
Test Time 2 hr.
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Frequently Asked Questions (FAQs)

The magnetic beads, that are shipped with the PrepSeq Residual DNA Sample Preparation Kit (Cat. No. 4413686), are clumped together and are difficult to get back into suspension. How can I fix this?

The beads are known to clump due to precipitation of the salts in the solution. These are highly concentrated chaotropic salt solutions. The only way to get the salts and the beads back into the solution is by incubating them at 37 degrees C for at least 10 minutes or until the solution is homogenous. Intermittent high speed vortexing helps expedite the process. To get beads stuck to the lid back into solution, we recommend inverting the tube and using high speed vortexing in a pulsing manner.
If incubating at 37 degrees C does not fix the issue, we recommend incubating at 45 degrees C.
What amount of DNA can be recovered with the PrepSEQ Residual DNA Sample Preparation Kit (Cat. No. 4413686)?

With the above kit, up to 5 ng of DNA can be recovered per 30 µl magnetic beads used per reaction. PrepSEQ is specially designed to recover lower amounts of DNA (in ng to sub femto‐gram levels).

For Research Use Only. Not for use in diagnostic procedures.