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Thermo Scientific™ POROS™ HQ Strong Anion Exchange HPLC Columns
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Catalog No. p-200156842
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Particle Size:
10 μm
20 μm
Dimensions:
2.1 x 100 mm
2.1 x 30 mm
4.6 x 100 mm
4.6 x 50 mm
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Catalog No. Particle Size Dimensions
1231216 10 μm 2.1 x 100 mm
1231224 10 μm 4.6 x 50 mm
1231226 10 μm 4.6 x 100 mm
1232212 20 μm 2.1 x 30 mm
1232224 20 μm 4.6 x 50 mm
1232226 20 μm 4.6 x 100 mm
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Catalog No. 1231216 Supplier Thermo Scientific™ Supplier No. 1231216
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Thermo Scientific™ POROS™ HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, High Performance Chromatography™ resins designed for the separation and purification of biomolecules.

POROS HQ is based on a quaternized polyethyleneimine functional group yielding a high capacity, High Performance Chromatography™ resins designed for the separation and purification of biomolecules.

10 micron particle size is used for the highest resolution chromatography, and is suitable for analytical to small scale preparative separation of biomolecules.

20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.

POROS High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.

  • Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
  • Chemical stability: Allows aggressive cleaning and sanitization
  • Enhanced biomolecule access: Provided via large pores, ranging between 500-10,000 Å
  • Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
  • Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed

In contrast to conventional chromatography media, POROS High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large 'throughpores' allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short 'diffusive' pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1000 to 5000 cm/hr compared to 50 to 360 cm/hr for conventional media.

Specifications

Column Format Analytical Column, Biocompatible PEEK
Diameter (Metric) 2.1 mm
Length (Metric) 100 mm
Max. Pressure 2500 psi (170 bar)
Particle Size 10 μm
pH 1-12
Pore Size 4000 Å
Quantity 1 Ea.
Temperature <60°C
Stationary Phase Quaternized Polyethylene Imine
Content And Storage Store at room temperature.
Product Line POROS
Binding Capacity ~55 mg/mL BSA at pH 8.0
Shipping Condition Room Temperature
Dimensions 2.1 x 100 mm
Packing Material Highly Crosslinked Styrene-Divinylbenzene polymer (ST-DVB)
Column Type Ion Exchange
Material PEEK
Type Prepacked Column
Technique Bio-LC
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