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Thermo Scientific™ POROS™ EP 20 μm Prepacked Immobilized Epoxide Affinity Columns
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Catalog No. p-200156844
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Catalog No. Quantity
1612224 1 Column
1612226 1 Column
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Catalog No. 1612224

Supplier: Thermo Scientific™ 1612224

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Thermo Scientific™ POROS™ Prepacked™ Immobilized Epoxide Affinity Column is based on an epoxide functional group designed for the immobilization of proteins through free amine groups.

POROS Immobilized Epoxide Affinity Column is based on an epoxide functional group designed for the immobilization of proteins through free amine groups. 20 micron particle size ensures high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS High Performance Chromatography™ Media and Pre-Packed Columns

  • A high performance chromatography resins for analytical to process scale separations.
  • Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
  • Chemical stability: Allows aggressive cleaning and sanitization
  • Enhanced biomolecule access: Provided via large pores, ranging between 500-10,000 Å
  • Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
  • Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed

In contrast to conventional chromatography media, POROS High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large 'throughpores' allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short 'diffusive' pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased–without compromising resolution or capacity. Separations can be achieved at 1000 to 5000 cm/hr compared to 50 to 360 cm/hr for conventional media.

Specifications

Capacity (Metric) 0.8 mL
Flow Rate 1000 to 5000 cm/hr.
Material PEEK
Matrix Polystyrenedivinylbenzene
Pore Size 2000 Å
Content And Storage Store at room temperature.
Diameter (Metric) 4.6 mm
For Use With (Equipment) HPLC
High-throughput Compatibility Not High-throughput Compatible (Manual)
Length (Metric) 50 mm
Shipping Condition Room Temperature
Sample Type Protein
Particle Size 20 μm
Product Line POROS
Quantity 1 Column
Type Column
Stationary Phase Epoxide
Format Column
Column Type Activated
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For Research Use Only. Not for use in diagnostic procedures.