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  6. Thermo Scientific™ POROS™ CaptureSelect™ FSH Affinity HPLC Columns

Thermo Scientific™ POROS™ CaptureSelect™ FSH Affinity HPLC Columns

Catalog No. 4481822
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Dimensions:
2.1 x 30 mm
4.6 x 100 mm
4.6 x 50 mm
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Catalog No. Dimensions
4481822 2.1 x 30 mm
4481824 4.6 x 50 mm
4481826 4.6 x 100 mm
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Catalog No. 4481822 Supplier Thermo Scientific™ Supplier No. 4481822
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Description

Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates.

Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS CaptureSelect FSH HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH).

Features of this class of chromotography columns:

  • Broad utility—POROS A, G, and CaptureSelect affinity ligands address most biological drug classes
  • Rapid—POROS resins offer fast quantitation or purification of immunoglobulins and fusion proteins
  • Hands-off operation—HPLC method can be automated for streamlined operation
  • Robust—delivers high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity

The combination of CaptureSelect FSH affinity ligand with POROS 20 μm resin provides a high flow-rate, high performance chromatography solution for the rapid quantitation and small-scale purification of FSH.

POROS high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in less than 3 minutes. Moreover, only minimal sample preparation (0.2 μm filtration) is required, and the eluted product is highly purified and suitable for further analysis, including mass spectrometry.

Automation

POROS affinity chromatography columns are suitable for use with HPLC systems, allowing for the automation of chromatography, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles

Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS affinity chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

Specifications

Length (Metric) 30 mm
Max. Pressure 180 bar
Particle Size 20 μm
Pore Size 2000 Å
Quantity 1 Column
Stationary Phase Follicle Stimulating Hormone affinity ligand, humanspecific
Content And Storage Column includes EZ grip fittings and column test certificate

Store at 4 °C. DO NOT FREEZE.
Product Line CaptureSelect, POROS
Binding Capacity 1.5 mg/mL resin
Elution Volume Max <100 μL
High-throughput Compatibility Automated Protocols
Sample Type Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures
Target Molecule Follicle Stimulating Hormone
Volume (Metric) 0.1 mL
Dimensions 2.1 x 30 mm
Column Type Affinity
Material PEEK
Type Column
Purification Target Follicle Stimulating Hormone
For Use With (Application) FPLC
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For Research Use Only. Not for use in diagnostic procedures.