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Invitrogen™ pLenti6.3/V5™-TOPO™ TA Cloning™ Kit

Catalog No. K531520
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Description

pLenti6.3/V5™-TOPO™ TA Cloning™ Kit

Invitrogen's™ pLenti6.3⁄V5™-TOPO™Cloning Kit is part of our ViraPower™ HiPerform™ Lentiviral Expression System (catalog K5310-00).

The pLenti6.3⁄V5™-TOPO™ -TA Cloning Kit contains the TOPO™- adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5™-TOPO™ for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5™-TOPO™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages
  • Stable expression
  • Long-term experiments
  • Accurate titer of functional virus
  • 5 minute, high-efficiency TOPO™ PCR cloning in three simple steps

    Key Features
  • HiPerform™ WPRE and cPPT elements
  • CMV promoter
  • V5 epitope tag at C terminus
  • Blasticidin selection

    Kit includes
  • A lacZ vector as a positive control, pLenti6.3⁄V5-GW⁄lacZ™
  • Stbl3™ competent cells

    For research use only. Not intended for any therapeutic or diagnostic use.

    • Specifications

    Cell Type Competent Cell
    Cloning Method TOPO-TA
    Constitutive or Inducible System Constitutive
    Content And Storage TOPO™ TA Cloning Reagents (Box 1)
    pLenti6.3?V5™-TOPO™ (5-10 ng⁄μL): 20 μL, -20°C
    pLenti6.3⁄V5-GW⁄lacZ™ Control vector (0.5 μg⁄μL): 20 μL, -20°C
    10X PCR Buffer: 100 μL, -20°C
    dNTP Mix: 10 μL, -20°C
    Salt Solution: 50 μL, -20°C
    CMV Forward Primer (100 ng⁄μL): 20 μL, -20°C
    V5 (C-term) Reverse Primer (100 ng⁄μL): 20 μL, -20°C
    Control PCR Template (50 ng⁄μL): 10 μL, -20°C
    Control PCR Primers (100 ng⁄μL): 10 μL, each -20°C
    Sterile Water: 1 mL

    One Shot™ Stbl3™ Chemically Competent E. coli (Box 2)
    Stbl3™ Cells: 21 x 50 μL, -80°C
    pUC19 control DNA (10 pg⁄μL): 50 μL, -80°C
    S.O.C. Medium: 6 mL, -80°C
    Delivery Type Lentiviral
    Format Kit
    Expression System ViraPower™ HiPerform™ Lentiviral Expression Systems
    For Use With (Application) Protein Expression
    Key Functions Viral Expression
    Product Line HiPerform, TOPO, ViraPower
    Product Type Cloning Kit
    Promoter CMV
    Protein Tag V5 Epitope Tag
    Quantity 1 kit
    Selection Agent (Eukaryotic) Blasticidin
    Selection Marker Promoter SV40 Promoter
    Vector pLenti, TOPO-TA Vector
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    For Research Use Only. Not for use in diagnostic procedures.