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Thermo Scientific™ PIP2:PS Lipid Kinase Substrate

Catalog No. PV5100
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400 nmol
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Catalog No. Quantity
PV5100 400 nmol
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Catalog No. PV5100 Supplier Thermo Scientific™ Supplier No. PV5100
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Description

Specifically optimized for use with the Class I PI3 kinases (PIK3CA, PIK3CB, PIK3CD, and PIK3CG), this substrate consists of lipid vesicles of a 1:19 molar ratio of phosphoinositol-4,5-bisphosphate (PIP2) with phosphotidylserine (PS).

Specifically optimized for use with the Class I PI3 kinases (PIK3CA, PIK3CB, PIK3CD, and PIK3CG), this substrate consists of lipid vesicles of a 1:19 molar ratio of phosphoinositol-4,5-bisphosphate (PIP2) with phosphotidylserine (PS).

For use with the Adapta™ Universal Kinase Assay (Cat. No. PV5099).

Specifications

Assay Entry Biochemical ADP accumulation
Chromagen Quantity 400nmol
Content And Storage PIP2:PS Lipid Kinase Substrate in 50 mM HEPES pH 7.5, 3 mM MgCl2, and 1 mM EGTA.

Store at -20°C.
Detection Method Fluorescence
For Use With (Application) Pharma and biopharma, protein assays and analysis, target and lead identification and validation, Class I PI3 kinases (PIK3CA, PIK3CB, PIK3CD, and PIK3CG), Adapta™ Universal Kinase Assay (PV5099), drug discovery and development, kinase biology, biochemical kinase assays, Adapta™ universal kinase assays, enzyme and protein activity assays, protein assays and analysis, kinase activity
Quantity 400 nmol
Substrate Phosphoinositol-4,5-bisphosphate (PIP2) lipid vesicles with phosphotidylserine (PS)
Substrate Properties Lipid-Based Substrate
Substrate Type Kinase Substrate
Target Enzyme Lipid Kinases
Gene ID (Entrez) 5290, 5294
Item Description PIP2:PS Lipid Kinase Substrate, 400nmol, consists of lipid vesicles of a 1:19 molar ratio of phosphoinositol-4,5-bisphosphate (PIP2) with phosphatidylserine (PS)
Product Type PIP2:PS Lipid Kinase Substrate
Shipping Condition Dry Ice
Storage Requirements –20°C
Target Entry PIK3CG, PIK3CD/PIK3R1, PIK3CA/PIK3R1
Validated Kinases PIK3CA, PIK3CG, PIK3CB, PIK3CD
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For Research Use Only. Not for use in diagnostic procedures.