Thermo Scientific™ Pierce Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed and can be used in conjunction with cell lysis buffers.Pierce Universal Nuclease for Cell Lysis is a genetically engineered endonuclease from Serratia marcescens. The enzyme is produced and purified from E. coli and consists of two identical 30-kDa subunits with two critical disulfide bonds. This indiscriminate endonuclease degrades single-stranded, double-stranded, linear and circular DNA and RNA and is effective over a wide range of temperatures and pH. This enzyme has very high specific activity (100-fold greater than DNase I) and has increased thermal stability compared to other nucleases. Pierce Universal Nuclease is ≥99% pure enzyme, is free of any measurable protease activity and is supplied at a concentration of 250U/μL. Pierce Universal Nuclease for Cell Lysis is identical in performance to Benzonase™ Nuclease by EMD Merck™.
Broad spectrum degrades all forms of DNA and RNA
Highest-quality enzyme nuclease is at least 99% pure, as tested by SDS-PAGE
Robust activity 100-fold greater specific activity than DNase I
Versatile can be used with a wide variety of cell lysis reagents
Use with B-PER, Y-PER or other commercial or homebrew cell lysis reagents and/or mechanical disruption to reduce viscosity in protein extracts; Remove DNA and RNA from recombinant protein preparations prior to downstream processing
|Pierce Universal Nuclease for Cell Lysis|
|At least 99% pure enzyme at 250U/uL in 50% glycerol (v/v), 20mM NaCl, 2mM MgCl2, 20mM Tris, pH 8.0|