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  6. Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format

Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format

Catalog No. PIA39268
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50 mg
10 x 2 mg
1 g
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Catalog No. Quantity
PIA39268 10 x 2 mg
PI22322 50 mg
PI22122 1 g
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Catalog No. PIA39268 Supplier Thermo Scientific™ Supplier No. A39268
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Description

Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.

Thermo Scientific™ Pierce Sulfo-SMCC is a very popular, water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane spacer arm (8.3 Ås).

  • Reactive groups: sulfo-NHS ester and maleimide
  • Reactive towards: amino and sulfhydryl groups
  • Water-soluble (compare to SMCC), so crosslinking can be done in physiologic solutions
  • High purity, crystalline reagent can be used to create high-purity maleimide-activated derivatives
  • Cyclohexane bridge confers added stability to the maleimide group making Sulfo-SMCC the ideal crosslinking agent for maleimide activation of proteins. Maleimide groups are stable for 64 hours in 0.1 M sodium phosphate buffer, pH 7 at 4°C.

Specifications

Chemical Reactivity Amine-Sulfhydryl
Cleavable No
Description Sulfo-SMCC
Molecular Weight (g/mol) 436.37
PEGylated No
Spacer Arm Length 8.3 Å
Content And Storage Upon receipt store desiccated at -20°C.
Cell Permeability No
Shipping Condition Ambient
Product Line No-Weigh
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety Maleimide, Sulfo-NHS Ester
Spacer Short (<10 Å)
Form Solid
Quantity 10 x 2 mg
Solubility Water
Format Standard, Single-use, Premium-grade
Water Soluble Yes
Product Type Crosslinker
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Frequently Asked Questions (FAQs)

Can you provide the shelf-life for Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)?

Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
What is the shelf life of Sulfo-SMCC (Cat. No. 22322)?

Sulfo-SMCC is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (desiccated at -20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).
How does a product with "No-Weigh" format reduce my concern over reagent stability?

The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html
We are using EDC to crosslink a protein onto carboxyl-modified beads. We would like to add in a linker molecule to extend the radius of beads to increase the surface area so more proteins can be immobilized. Is this possible?

To provide a linker between the beads and the target protein, you would need a molecule with a primary amine (to conjugate to the carboxyl group on the beads with EDC) on one end and another reactive group on the other (to conjugate to the protein). For instance, sulfo-SMCC (Cat. No. A39268) could be used in conjunction with PDPH (Cat. No. 22301):
The method is outlined below:
1. Conjugate the target protein to the NHS ester (amine-reactive) group of Sulfo-SMCC. This will produce a maleimide-activated target protein via its primary amines (lysines and N-terminus).
2. Remove the excess sulfo-SMCC by desalting or dialysis.
3. React the maleimide-activated target protein to PDPH via sulfhydryls. (Lysines and N-terminus now are connected to hydrazide (amine) group with an extended linker)
4. Remove excess PDPH by desalting or dialysis.
5. React the hydrazide-activated target protein with carboxyl beads in the presence of EDC.
6. Use appropriate buffer to rinse off excess target protein from the carboxyl beads.

For Research Use Only. Not for use in diagnostic procedures.