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Thermo Scientific™ Pierce™ Streptavidin Plus UltraLink™ Resin

Catalog No. PI53117
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Catalog No. PI53117 Supplier Thermo Scientific™ Supplier No. 53117
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Description

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

Thermo Scientific™ Pierce Streptavidin Resins comprise a wide selection of high-quality affinity beads and columns for nearly any kind of biotin-based purification procedure.

Additional Information and Online Ordering

Pierce Streptavidin Agarose and UltraLink Resins supply immobilized streptavidin biotin-binding protein in different resin formats, binding capacities and package sizes to suit nearly any immunoprecipitation or affinity purification procedure involving biotinylated molecules. The products use a recombinant form of streptavidin with a mass of 53 kDa and a near-neutral pI. The resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in Streptavidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11. The products are excellent choices for a variety of small- or large-scale affinity purification applications involving biotinylated macromolecules, including separation of biotinylated molecules from samples and immunoprecipitation of antigens using biotin-labeled antibodies.

Highlights:

  • Proven coupling chemistries – the methods used to prepare these resins are superior to typical cyanogen bromide (CNBr) immobilization, providing high functionality, low nonspecific binding and excellent leach-resistance

  • Versatile resins – Agarose (polysaccharide-based) and UltraLink™ Biosupport (acrylamide-based) resins provide two different options for particular experimental conditions and are compatible with gravity-flow, spin and FPLC methods

  • Available in multiple formats – choose from two resin types, regular or high capacity preparations, and small or large-scale package sizes (bulk sizes are also available)

  • Superior performance – High Capacity Resin has 2 to 3 times higher binding capacity than alternative supplier products, allowing use of small amounts of resin for experiments

Item-Specific Features:

  • Agarose Resins are crosslinked 6% beaded agarose
  • UltraLink Resins beaded acrylamide support (UltraLink Biosupport)
  • Plus and High Capacity Resins have higher loading (i.e. density) of immobilized streptavidin protein than the regular forms

Recommended for:

Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies; Cell-surface labeling with biotinylation reagents, followed by precipitation with immobilized streptavidin; Purification of cell-surface glycoproteins using biotinylated Concanavalin A; Recovery of single-stranded DNA for dideoxy sequencing

Specifications

Capacity (Metric) 10 mg Biotinylated BSA Binding per mL Resin
Content And Storage Upon receipt store at 4°C. Product is shipped at ambient temperature.
Particle Size 45 to 165 μm
Product Line Pierce, UltraLink
Quantity 5 mL
Protein Form Recombinant
Target Biotin-Tagged Molecule
Type Resin
Stationary Phase Streptavidin
Sufficient For Binding ≥/= 48 μg Biotin/mL Resin (≥/= 4 mg Biotinylated BSA/mL Resin)
Format Bottle
Column Type Affinity, UltraLink™ Resin
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Frequently Asked Questions (FAQs)

When should the UltraLink Immobilized Streptavidin Plus Support be used in place of streptavidin immobilized on agarose?

UltraLink Immobilized Streptavidin Plus Support should be used when you are planning to perform anything other than gravity filtration with a column. Streptavidin Plus UltraLink Resin is often preferred for immunoprecipitation because the white appearance of the support allows the pellet to be visualized more easily than the translucent pellet of agarose supports.
What is the biotin-binding capacity for UltraLink Biosupport Medium?

The biotin-binding capacity is >3 mg biotinylated BSA/ml of resin, or >120 nMoles biotin/mL of resin which is equilvalent to approximately 30 µg of biotin or 30 units of biotin/ml.
What is the affinity of streptavidin for biotin in the Thermo Scientific Streptavidin Plus Ultralink Resin kit?

The binding affinity of streptavidin is similar to that of avidin, which is Ka = 10^15/M.
What is UltraLink Biosupport Medium?

UltraLink Biosupport Medium is stronger than agarose, being capable of withstanding 100 p.s.i. The support starts with an azlactone ring that opens upon immobilization of a ligand to form a five-molecule spacer arm.
What can prevent biotin from binding to the immobilized streptavidin when using the Thermo Scientific Streptavidin Plus Ultralink Resin?

Steric hindrance can interfere with the binding. As with avidin, the double ring structure of biotin must fit 9 angstroms into the streptavidin molecule for binding to occur. Competitive inhibition by endogenous biotin may also interfere with binding of your biotinylated ligand.
Can I make an affinity support using the Thermo Scientific Streptavidin Plus Ultralink Resin?

Yes. Biotinylate your capture ligand and incubate it with the support. The biotin will bind the ligand to the support. The support can now be used to pull down molecules with an affinity for the ligand, and these molecules can be eluted using standard elution buffers without fear of contamination from the biotinylated ligand.
After my biotinylated ligand is bound to the support when using the Thermo Scientific Streptavidin Plus Ultralink Resin, what conditions are needed to elute it from the support?

The conditions necessary for elution are very harsh. To elute the biotin, the streptavidin must be irreversibly denatured via one of two common methods: boiling in SDS-PAGE sample buffer or incubation with 8 M guanidine chloride, pH 1.5. When working with a small biotinylated ligand, sometimes even the latter elution method is not strong enough to recover the entire sample. With either of these elution methods, the support cannot be reused due to the destruction of the streptavidin.

For Research Use Only. Not for use in diagnostic procedures.