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  6. Thermo Scientific™ Pierce™ Streptavidin Agarose

Thermo Scientific™ Pierce™ Streptavidin Agarose

Catalog No. PI20353
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PI20353 10 mL
PI20347 2 mL
PI20349 5 mL
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Catalog No. PI20353 Supplier Thermo Scientific™ Supplier No. LSG20353
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Description

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

Thermo Scientific™ Pierce Streptavidin Resins comprise a wide selection of high-quality affinity beads and columns for nearly any kind of biotin-based purification procedure.

Additional Information and Online Ordering

Pierce Streptavidin Agarose and UltraLink Resins supply immobilized streptavidin biotin-binding protein in different resin formats, binding capacities and package sizes to suit nearly any immunoprecipitation or affinity purification procedure involving biotinylated molecules. The products use a recombinant form of streptavidin with a mass of 53 kDa and a near-neutral pI. The resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in Streptavidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11. The products are excellent choices for a variety of small- or large-scale affinity purification applications involving biotinylated macromolecules, including separation of biotinylated molecules from samples and immunoprecipitation of antigens using biotin-labeled antibodies.

Highlights:

  • Proven coupling chemistries – the methods used to prepare these resins are superior to typical cyanogen bromide (CNBr) immobilization, providing high functionality, low nonspecific binding and excellent leach-resistance

  • Versatile resins – Agarose (polysaccharide-based) and UltraLink™ Biosupport (acrylamide-based) resins provide two different options for particular experimental conditions and are compatible with gravity-flow, spin and FPLC methods

  • Available in multiple formats – choose from two resin types, regular or high capacity preparations, and small or large-scale package sizes (bulk sizes are also available)

  • Superior performance – High Capacity Resin has 2 to 3 times higher binding capacity than alternative supplier products, allowing use of small amounts of resin for experiments

Item-Specific Features:

  • Agarose Resins are crosslinked 6% beaded agarose
  • UltraLink Resins beaded acrylamide support (UltraLink Biosupport)
  • Plus and High Capacity Resins have higher loading (i.e. density) of immobilized streptavidin protein than the regular forms

Recommended for:

Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies; Cell-surface labeling with biotinylation reagents, followed by precipitation with immobilized streptavidin; Purification of cell-surface glycoproteins using biotinylated Concanavalin A; Recovery of single-stranded DNA for dideoxy sequencing

Specifications

Capacity (Metric) 1 to 3 mg Biotinylated BSA Binding per mL Resin
Content And Storage Upon receipt store at 4°C. Do not freeze the resin. Product is shipped at ambient temperature.
Particle Size 45 to 165 μm
Product Line Pierce
Quantity 10 mL
Protein Form Recombinant
Target Biotin-Tagged Molecule
Type Agarose
Stationary Phase Streptavidin
Sufficient For Binding 15 to 28 μg Biotin/mL Resin (1 to 3 mg Biotinylated BSA/mL Resin)
Format Bottle
Column Type Affinity, Agarose Resin
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Frequently Asked Questions (FAQs)

What is the particle size of the Pierce Streptavidin Agarose beads (Cat. No. 20347, 20353, 20349)?

The average particle size for the Pierce Streptavidin Agarose beads is 45 to 165 µm.
What is the recommend/ideal antigen:antibody ratio when using the Pierce Streptavidin Agarose (Cat. No. 20347) for doing an immunoprecipitation (with pre-incubating antigen and antibody)?

The antigen:antibody ratio will need to be determined empirically since one cannot be sure of the amount of specific antibody against the antigen present in the plasma. The starting point is the binding capacity of the High Capacity Streptavidin Agarose Resin (Cat. No. 20347) which is 1-3 mg of biotinylated BSA/mL of settled resin. The binding capacity will vary depending on the molecular weight of the biotinylated antigen. Let's assume the molecular weight of the antigen is close to that of BSA (66.4 kDa), you can use an average of 2 mg antibody or antigen/mL of resin to do the calculation. Determine how much resin they want to use and calculate how much biotinylated protein is needed and then empirically determine how much plasma is needed.

What is the shelf life for Pierce Streptavidin Agarose?

We guarantee the functionality of the product for one year from the date of shipment, if stored as recommended.
How many biotin binding-sites are there per streptavidin molecule?

Streptavidin is a protein composed of four identical subunits, each containing a high affinity binding site for biotin (K-D = 10 -15 M) . Streptavidin has the same biotin binding properties as avidin, but it has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low non-specific binding.

For Research Use Only. Not for use in diagnostic procedures.