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Thermo Scientific™ Sodium Dodecyl Sulfate (SDS), C12

Catalog No. PI28312
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500 g
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PI28312 500 g
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Catalog No. PI28312 Supplier Thermo Scientific™ Supplier No. LSG28312
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Description

Highly purified form of SDS especially suited for protein experiments requiring tightly controlled solubility parameters.

Thermo Scientific™ Sodium Dodecyl Sulfate (C12) is a highly purified form of SDS that is especially suited for protein experiments requiring tightly controlled solubility parameters.

Sodium dodecyl sulfate (SDS) is an alkyl sulfate, anionic detergent used frequently in protein electrophoresis and protein solubilization methods. It is a component of many cell lysis buffer formulations, including RIPA buffer. Typical SDS or lauryl sulfate preparations are mixture of several different chain lengths (C10 to C18). However, protein renaturation and other specialized methods require very specific surfactant and buffer conditions. This particular formulation of SDS is highly purified to eliminate nearly all secondary chain lengths besides C12.

Highlights:

  • Popular anionic detergent for protein electrophoresis and solubilization
  • Component of many cell lysis buffer formulations
  • Specially purified formulation is >98% C12 alkyl sulfate
  • Contains low levels of hexadecyl sulfate (C16), which inhibits protein renaturation

Detergent properties (pure C12):

  • Molecular Weight: 288.5
  • Detergent Class: Ionic (anionic)
  • Aggregation Number: 62
  • Micelle Molecular Weight: 18,000
  • Critical Micelle Concentration (CMC): 6 to 8mM (0.1728 to 0.2304%, w/v)
  • Cloud Point: >100°C
  • Dialyzable: No

Specifications

Product Type Detergent
Content And Storage Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20°C to 25°C.
Form Powder
Reagent Type Detergent (Pure)
Quantity 500 g

Frequently Asked Questions (FAQs)

Are detergents denaturing or non-denaturing with respect to protein structure?

Ionic detergents, or those that carry a charge, are the most likely to be denaturing to proteins. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking protein-protein interactions through changes in the three-dimensional structure of the proteins. Nondenaturing detergents can be divided into nonionic detergents (i.e., Triton X-100), bile salts (i.e., cholate), and zwitterionic detergents (i.e., CHAPS).
What are detergents?

Detergents are amphipathic molecules containing both a nonpolar “tail” having aliphatic or aromatic character, and a polar “head”. The ionic character of the polar head group forms the basis for broad classification of detergents as ionic, nonionic, or zwitterionic.

For Research Use Only. Not for use in diagnostic procedures.