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Thermo Scientific™ PEG4-SPDP (PEGylated, long-chain SPDP crosslinker)

Catalog No. pi26128
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Quantity:
100 mg
Catalog No. Quantity
PI26128 100 mg
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Catalog No. PI26128

Supplier: Thermo Scientific™ LSG26128

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Description

Long (25.7A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 4-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.

Thermo Scientific™ Pierce PEG4-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a 4-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.

SPDP reagents have an amine-reactive N-hydroxysuccinimide (NHS) ester at one end and a suflhydryl-reactive 2-pyridyldithio group at the opposite end. PEG4-SPDP has a 4-unit polyethylene glycol spacer arm, which confers greater solubility to the crosslinker and linked proteins compared to crosslinkers having only hydrocarbon spacers. Pyridyldithiol reagents produce disulfide-containing linkages that can be cleaved with reducing agents such as dithiothreitol (DTT). Crosslinking experiments with SPDP reagents are not limited to those involving proteins. Any of a variety of molecules with primary amines and sulfhydryl groups can be modified or crosslinked using an SPDP reagent.

Highlights:

  • Efficient amine conjugation – NHS ester provides effective conjugation to lysines, N-termini of peptides and other primary amines

  • Reversible sulfhydryl conjugation – pyridyldithio group enables disulfide linkage with reduced cysteines and other sulfhydryl groups, providing a stable but cleavable bond

  • Polyethylene glycol – PEG groups are flexible, non-immunogenic, hydrophilic, and often enhance the solubility of attached molecules

  • Spacer arm – connects primary amines and sulfhydryl targets at distances up to 25.7 angstroms

Specifications:

  • Chemical name (simplified): 2-pyridyldithiol-tetraoxatetradecane-N-hydroxysuccinimide
  • Chemical formula: C23H33N3O9S2
  • Molecular weight: 559.65
  • Spacer arm: 25.7 angstroms
  • Form: straw to amber colored oil
  • Solubility: dissolve 2mg in 179μL DMSO or DMF to make 20mM stock for each use

Specifications

Chemical Reactivity Amine-Sulfhydryl
Cleavable By Thiols
Description PEG4-SPDP
Molecular Weight (g/mol) 559.65
PEGylated Yes
Spacer Arm Length 25.7 Å
Content And Storage Upon receipt store at -20°C protected from moisture.
Cell Permeability No
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety NHS Ester, Pyridyldisulfide
Spacer Medium (10 to 30 Å)
Form Liquid
Quantity 100 mg
Solubility Water
Format Standard
Water Soluble Yes
Product Type Crosslinker
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For Research Use Only. Not for use in diagnostic procedures.