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Thermo Scientific™ PDPH (3-(2-pyridyldithio)propionyl hydrazide)

Catalog No. PI22301
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50 mg
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Catalog No. Quantity
PI22301 50 mg
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Catalog No. PI22301 Supplier Thermo Scientific™ Supplier No. LSG22301
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Description

Crosslink sulfhydryls to periodate-treated glycoprotein sugars via cleavable disulfide bonds using this short crosslinker containing pyridyldithiol and hydrazide groups.

Thermo Scientific™ Pierce PDPH is a short, pyridyldithiol-and-hydrazide crosslinker for reversibly conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Highlights:

  • Pyridyldisulfide group provides a cleavable disulfide bond
  • Oxidized carbohydrate-reactive hydrazide
  • Reactive groups: pyridyldisulfide and hydrazide
  • Reactive toward: sulfhydryl groups and carbohydrate
  • Use with EDC for reactivity toward carboxyl groups

Specifications

Chemical Reactivity Carbohydrate-Sulfhydryl
Cleavable By Thiols
Description PDPH
Molecular Weight (g/mol) 229.32
PEGylated No
Spacer Arm Length 9.2 Å
Content And Storage Upon receipt store desiccated at 4°C.
Cell Permeability Yes
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety Hydrazide, Pyridyldisulfide
Spacer Short (<10 Å)
Form Powder
Quantity 50 mg
Solubility DMF, DMSO
Format Standard
Water Soluble No
Product Type Crosslinker
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Frequently Asked Questions (FAQs)

Can you provide the shelf-life for PDPH (3-(2-pyridyldithio)propionyl hydrazide)?

PDPH (3-(2-pyridyldithio)propionyl hydrazide) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
We are using EDC to crosslink a protein onto carboxyl-modified beads. We would like to add in a linker molecule to extend the radius of beads to increase the surface area so more proteins can be immobilized. Is this possible?

To provide a linker between the beads and the target protein, you would need a molecule with a primary amine (to conjugate to the carboxyl group on the beads with EDC) on one end and another reactive group on the other (to conjugate to the protein). For instance, sulfo-SMCC (Cat. No. A39268) could be used in conjunction with PDPH (Cat. No. 22301):
The method is outlined below:
1. Conjugate the target protein to the NHS ester (amine-reactive) group of Sulfo-SMCC. This will produce a maleimide-activated target protein via its primary amines (lysines and N-terminus).
2. Remove the excess sulfo-SMCC by desalting or dialysis.
3. React the maleimide-activated target protein to PDPH via sulfhydryls. (Lysines and N-terminus now are connected to hydrazide (amine) group with an extended linker)
4. Remove excess PDPH by desalting or dialysis.
5. React the hydrazide-activated target protein with carboxyl beads in the presence of EDC.
6. Use appropriate buffer to rinse off excess target protein from the carboxyl beads.

For Research Use Only. Not for use in diagnostic procedures.