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Thermo Scientific™ Pierce™ Organelle Enrichment Kits


Enrich for lysosomes, peroxisomes and nuclei from cells and tissue with these organelle-specific subcellular fractionation kits.

Manufacturer: thermo scientific™  89839

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Catalog No. PI89839

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Description & Specifications

Specifications

Formulation Four-component kit
Description Pierce Lysosome Enrichment Kit for Tissues and Cultured Cells
Quantity 230mL kit
Source Material Cultured mammalian cells, Animal tissue
Kit Contents Lysosome Enrichment Reagent A, 90mL
Sufficient For 25 samples each containing 50 to 200mg of cells or tissue

Perform enrichment of intact organelles including lysosomes, peroxisomes and nuclei from cells and tissue with Thermo Scientific™ Organelle Enrichment Kits.

These three organelle-specific kits provide the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation. Subcellular fractionation simplifies otherwise complex protein mixtures that result from whole cell lysis, thereby facilitating proteomic analysis of specific organelle targets and pathways. Each kit uses density gradient centrifugation to separate organelles from contaminating cellular structures. The isolated organelles may be used for a number of downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localization studies.

Highlights:

  • Efficient and easy to use – kit reagents and gradient centrifugation separate organelles from contaminating structures

  • Compatible – prepare samples for downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localization studies

  • Complete – kits contain sufficient material for 25 applications

Includes:

Organelle-specific Reagents A and B, Optiprep™ Cell Separation Media, and phosphate-buffered saline

Requires:

Ultracentrifuge, refrigerated microcentrifuge, vortex mixer, Dounce tissue grinder, sonicator