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Thermo Scientific™ Lysosome Enrichment Kit for Tissues and Cultured Cells
Enrich for lysosomes, peroxisomes and nuclei from cells and tissue with these organelle-specific subcellular fractionation kits.
Supplier: Thermo Scientific™ 89839
Description
Perform enrichment of intact organelles including lysosomes, peroxisomes and nuclei from cells and tissue with Thermo Scientific™ Organelle Enrichment Kits.
These three organelle-specific kits provide the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation. Subcellular fractionation simplifies otherwise complex protein mixtures that result from whole cell lysis, thereby facilitating proteomic analysis of specific organelle targets and pathways. Each kit uses density gradient centrifugation to separate organelles from contaminating cellular structures. The isolated organelles may be used for a number of downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localization studies.Highlights:
Efficient and easy to use – kit reagents and gradient centrifugation separate organelles from contaminating structures
Compatible – prepare samples for downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localization studies
Complete – kits contain sufficient material for 25 applications
Includes:
Organelle-specific Reagents A and B, Optiprep™ Cell Separation Media, and phosphate-buffered saline
Requires:
Ultracentrifuge, refrigerated microcentrifuge, vortex mixer, Dounce tissue grinder, sonicator
Specifications
| Lysosome Enrichment Kit | |
| Liquid | |
| 25 Samples of 50 to 200 mg Cells or Tissues Each |
| Upon receipt store at 4°C. Product shipped at ambient temperature | |
| Organelle Isolation, Subcellular Fractionation | |
| 230 mL |
For Research Use Only. Not for use in diagnostic procedures.