missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)

Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.

Manufacturer:  Thermo Scientific™ PI34055

Catalog No. PI34055



Description

Description

Thermo Scientific™ Pierce ONPG Substrate is the preferred colorimetric substrate for ELISA applications involving β-galactosidase (β-Gal) as the reporter enzyme.

ONPG (ω-nitrophenyl-β-D-galactopyranoside) is the preferred colorimetric substrate for ELISA applications involving β-galactosidase (β-Gal) reporter enzyme. The enzyme-substrate reaction yields a yellow product with an absorbance maximum at 420nm but whose intensity is also very high at 405nm (matching a common plate reader filter set).

Highlights:

  • Product formed is completely soluble
  • High extinction coefficient at 405nm
  • Substrate yields a yellow product that is easily detectable in the visual range after stopping the reaction with 1M sodium carbonate
Specifications

Specifications

Chemical Substrate
Colorimetric
Beta-Gal Substrate
Upon receipt store at -20°C protected from moisture and light. Product is shipped at ambient temperature.
SDS
Documents