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Thermo Scientific™ Mitochondria Isolation Kit for Tissue

Catalog No. PI89801
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115 mL
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PI89801 115 mL
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Catalog No. PI89801 Supplier Thermo Scientific™ Supplier No. 89801
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Description

Isolate intact mitochondria from soft and hard tissue samples in about an hour with this versatile, benchtop microcentrifuge-based fractionation kit.

Isolate intact mitochondria from soft and hard tissue samples in about an hour with Thermo Scientific™ Mitochondria Isolation Kit for Tissue.

The kit provides for two methods for mitochondria isolation. The first method uses a unique reagent-based procedure that enables simultaneous multi-sample processing. The second method relies on traditional Dounce homogenization for tissue disruption and subsequent isolation of the organelle. Both procedures use differential centrifugation to separate the intact mitochondria using a bench-top microcentrifuge and are completed in less than 60 minutes. In addition, both procedures have been optimized for maximum yield of mitochondria with minimal damage to integrity. The isolated mitochondria may be used for a number of downstream applications, including 1D and 2D Western blotting and protein profiling using mass spectroscopy.

Highlights:

  • Quick and convenient – isolate intact mitochondria in less than 60 minutes

  • Versatile – offers two methods of isolation from both soft- and hard-tissue samples

  • Multi-sample format – reagent-based approach enables simultaneous processing of multiple samples

  • Optional alternate method – reagents and protocol included for the traditional Dounce homogenization procedure with fewer required strokes

  • Benchtop-compatible – both procedures are performed using a microcentrifuge tube

Includes:

Mitochondria Isolation Reagents A, B and C; bovine serum albumin and a phosphate-buffered saline pack

Specifications

Product Type Cell Isolation Kit
Content And Storage Upon receipt store kit at 4°C. Product is shipped at ambient temperature.
Form Liquid
Reagent Type Organelle Isolation, Subcellular Fractionation
Sufficient For 50 Isolations
Quantity 115 mL

Frequently Asked Questions (FAQs)

Can you provide the shelf-life for the Mitochondria Isolation Kit for Tissues?

The Mitochondria Isolation Kit for Tissues is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
What kits do you offer for cell fractionation?

We offer the following kits for cell fractionation:

Mem-PER Plus Membrane Protein Extraction Kit, Cat. No. 89842
NE-PER Nuclear and Cytoplasmic Extraction Reagents, Cat. No. 78835
Subcellular Protein Fractionation Kit for Cultured Cells, Cat. No. 78840
Subcellular Protein Fractionation Kit for Tissues, Cat. No. 87790
Mitochondrial Isolation Kit for Cultured Cells, Cat. No. 89874
Mitochondrial Isolation kit for Tissue, Cat. No. 89801
Lysosome Enrichment Kit for Tissues and Cultured Cells, Cat. No. 89839
Why is cell fractionation important?

Isolation of subcellular fractions and concentration of low-abundantce proteins allows for more efficient identification and study of the proteins of interest.

For Research Use Only. Not for use in diagnostic procedures.