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Thermo Scientific™ Micrococcal Nuclease Solution (≥100 U/μL)

Catalog No. PI88216
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Quantity:
150 μL
Catalog No. Quantity
PI88216 150 μL
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Catalog No. PI88216

Supplier: Thermo Scientific™ LSG88216

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Description

Remove nucleic acids from cell lysates, release chromatin-bound proteins and shear chromatin for chromatin immunoprecipitation (ChIP) using micrococcal nuclease.

Thermo Scientific™ Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

This micrococcal nuclease is a stable liquid form of the enzyme derived from Staphylococcus aureus. Micrococcal nuclease (MNase) exhibits exo- and endo-5'-phosphodiesterase activities against DNA and RNA. This enzyme digests double-stranded, single-stranded, circular and linear nucleic acids. The highest activity is toward single-stranded nucleic acid substrates with preference for AT- or AU-rich regions. Enzymatic activity occurs at pH 7 to 10 and is strictly dependent on calcium for the digestion of RNA and DNA substrates. Micrococcal nuclease is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

Highlights:

  • Digests nucleic acids (DNA and RNA)

  • Effective for double-stranded, single-stranded, circular and linear nucleic acids
  • Active in neutral to alkaline buffer conditions containing divalent calcium ions
  • Supplied at ≥100 units/μL in 10mM Tris-HCl, pH 7.5, 50mM NaCl, 1mM EDTA, 50% glycerol

Compatible with:

Thermo Scientific Subcellular Protein Fractionation Kit (Part No. 78840); Thermo Scientific Pierce ChIP Kit (Part No. 26156)

Recommended for:

Enzymatic DNA shearing for chromatin immunoprecipitation assays (ChIP); Removal of unwanted genomic or organellar nucleic acids from cell lysates; Degradation of nucleic acids in subcellular fractionation procedures

Specifications

Product Type Cell Lysis Enzyme
Content And Storage Upon receipt store at -20°C. Product is shipped with dry ice.
Form Liquid
Reagent Type Enzyme for Cell Lysis
Quantity 150 μL

For Research Use Only. Not for use in diagnostic procedures.