Thermo Scientific™ Pierce Microcentrifuge Spin Cups and Spin Columns are convenient tools for manipulating small volumes of affinity supports for protein purification.
These microcentrifuge spin cups and spin columns are convenient tools for manipulating small volumes of affinity supports (5 to 500μL) for immunoprecipitation (IP) and pull-down purification assays. Five styles of polypropylene columns and matching collection tubes offer a variety of options to suit particular types of experimental needs. Add the affinity resin and sample to one of the columns and use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin columns allow you to affinity-purify more protein in less time.
Highlights:
Efficient washing of samples means fewer washes are needed to remove contaminating proteins
Efficient elution of samples means better recovery of immunoprecipitation and assay products
Filter cups and columns eliminate contamination of eluted samples with affinity resin
Filter cups and columns eliminate the need to decant supernatant from pelleted debris or resin
Spin protocols drastically reduce the time required for small-scale affinity protocols
Low protein-binding polypropylene column construction minimizes nonspecific binding
Spin Cups-Paper Filter with Collection Tubes (Part No. 69700):
Column volume: 600μL
Resin volume: 20 to 300μL
Filter type: paper, 10μm pore size
Cap type: collection tube cap fits onto inserted spin cup
Spin Cups-Cellulose Acetate Filter with Collection Tubes (Part No. 69702):
Column volume: 800μL
Resin volume: 20 to 400μL
Filter type: cellulose acetate, 0.45μm pore size
Cap type: collection tube cap fits onto inserted spin cup
Spin ColumnsScrew Cap with Luer-Lok
Spin Columns-Screw Cap (Part No. 69705):
Column Volume: 900μL
Resin volume: 20 to 400μL
Filter type: polyethylene filter, 10μm pore size; small and large frit options for different sample sizes
Cap type: O-ring screw top caps; press-in bottom plugs
Luer-Lok Adapters enable use with syringe-based kits
Spin Columns-Snap Cap with Collection Tubes (Part No. 69725):
Column volume: 1000μL
Resin volume: 20 to 500μL
Filter type: polyethylene filter, 30μm (20 to 50μm) pore size
Cap type: snap cap on column (no cap on collection tube); press-on bottom caps
Micro-Spin Columns (Part No. 89879):
Column volume: 400μL
Resin volume: 5 to 100μL
Filter type: polyethylene filter, 30μm (20 to 50μm) pore size
Cap type: O-ring screw top caps; press-on bottom caps
