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Thermo Scientific™ Pierce™ Mammalian β-Galactosidase Assay Kit


Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.

Manufacturer: thermo scientific™  75705

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Catalog No. PI75705

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Description & Specifications

Specifications

Description Pierce beta-Galactosidase Assay Reagent
Formulation Proprietary aqueous solution, pH 7.3
Quantity 25mL
Sufficient For 250 microplate wells of adherent cells

Perform a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity using Thermo Scientific™ Mammalian β-Galactosidase Assay Kit.

The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Highlights:

  • Choose stand-alone assay reagent or complete kit for cell lysis and detection
  • Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
  • Adaptable to 96-well microplates or tube assay formats
  • Perform homogeneous screening assays or measure activity secondarily after cell lysis
  • Also compatiible with prepared lysates from other species such as bacteria

Includes:

Stand-alone Thermo Scientific Pierce™ β-Galactosidase Assay Reagent, Thermo Scientific M-PER Mammalian Protein Extraction Reagent (Part No. 78503), and ready-to-use Stop Solution