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Thermo Scientific™ Pierce™ Magnetic RNA-Protein Pull-Down Kit

Utilize a streamlined, robust method to enrich protein-RNA interactions using end-labeled RNA as the bait with Thermo Scientific™ Pierce Magnetic RNA-Protein Pull-Down Kit.

Supplier:  Thermo Scientific™ LSG20164

Catalog No. PI20164


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Description
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Specifications

Specifications

1 mL
Sufficient For: 20 RNA-protein complex pull-down reactions

Slurry: 10mg/mL, 1% solids
• Pierce Nucleic Acid-Compatible Streptavidin Magnetic Beads, 1 mL (store at 4°C)
• RNA Capture Buffer (1X), 10 mL (store at 4°C)
• Tris (20 mM, pH 7.5), 5 mL (store at 4°C)
• Protein-RNA Binding Buffer (10X), 1 mL (store at 4°C)
• Wash Buffer (1X), 10 mL (store at 4°C)
• Biotin Elution Buffer, 1.5 mL (store at 4°C)
• HuR Monoclonal Antibody (Mouse), 50 μL (store at 4°C)
• Positive RNA Control (AR RNA), 250 pmol (store at -20°C)
• Negative RNA Control (polyA25 RNA), 250 pmol (store at -20°C)
• Pierce RNA 3' End Desthiobiotinylation Kit (part number 20163; store at -20°C)
20 reactions kit
20 Reactions
50 pmol of RNA per Reaction
Pierce™
RNA-Protein Pull Down Kit
Kit
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For Research Use Only. Not for use in diagnostic procedures.