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Thermo Scientific™ Pierce™ Kinase Enrichment Kit with ATP Probe

Catalog No. PI88310
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PI88310 16 reactions
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Catalog No. PI88310 Supplier Thermo Scientific™ Supplier No. 88310

Description

Desthiobiotin-ATP and -ADP probes to label, affinity purify and measure the active site of ATPases (kinases), including chaperones and metabolic enzymes.

Thermo Scientific™ Pierce Kinase Enrichment Kits utilize ActivX™ ATP or ADP Probes to covalently label the active site of ATPases, including chaperones and metabolic enzymes, to enable their selective enrichment using a desthiobiotin tag.

These products, based on ActivX ATP and ADP Probes, feature an amine-reactive nucleotide analog and a desthiobiotin (biotin analog) tag that facilitates selective labeling of lysines in the kinase active site and then subsequent enrichment and recovery of labeled protein. These features allow identification and profiling of target enzyme classes across samples or assessment of the specificity and affinity of enzyme inhibitors.

Highlights:

  • Specific – label only the conserved active-site lysines of nucleotide-binding proteins

  • Flexible – use for in vitro labeling of ATPase enzymes derived from cells or tissues

  • Compatible – use with Western blot or mass spectrometry (MS) workflows

Recommended for:

Profile small-molecule binding affinities and active-site inhibition in a dose-dependent manner; Identify dozens to hundreds of inhibitor targets and off-targets from tissues, cells and subcellular proteomes; Enrichment of enzymes based on function.

Specifications

Formulation Multi-component kit with Desthiobiotin-ATP Probe
Content And Storage ActivX Desthiobiotin-ATP Probe is shipped separately with dry ice and stored at -80°C upon receipt. All other kit components are shipped at ambient temperature and stored at 4°C upon receipt.
Assay Protein Quantitation Assay
Detection Method Chemiluminescence, Mass Spectrometry, Western Blot
Substrate Type Kinase Substrate
Product Line ActivX, Pierce
Quantity 16 reactions
For Use With (Application) Pull-Down
Target Kinases (Multiple), Phosphodiesterases, ATPases, GTPases, Phosphatases
Type Kinase Enrichment Kit
Label or Dye Biotin & Other Hapten
Format Tube, Spin Column
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Frequently Asked Questions (FAQs)

Can I isolate my proteins based on their post-translational modification (PTM) or active binding site?

Enrichment of specific proteins or protein complexes can most easily be accomplished by using target-specific immobilized antibody or PTM specific affinity binding. We offer several kits targeting phosphorylated proteins, alpha- linked mannose and terminal glucose residues, N-acetyl glucosamine, polyubiquinated proteins, ATP binding sites, and active serine hydrolase enzymes. Choose the right protein enrichment kit for your application using the following link (http://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-purification/protein-enrichment.html).

For Research Use Only. Not for use in diagnostic procedures.