Thermo Scientific™ Pierce™ Iminobiotin and Biotin Resins
Agarose beads of immobilized biotin (and iminobiotin) to strongly (or reversibly) bind and remove (or purify) streptavidin or avidin proteins and conjugates from samples.
Manufacturer: Thermo Scientific™ PI20221
Thermo Scientific™ Pierce Iminobiotin and Biotin Resins provide for unusual affinity purification experiments involving clean-up or removal of streptavidin or avidin protein components.Pierce Biotin Agarose and Iminobiotin Agarose are beaded agarose supports onto which biotin and iminobiotin moieties have been covalently immobilized. Typical avidin-biotin affinity procedures use immobilized avidin or streptavidin proteins to capture biotin-tagged molecules from a sample. Occasionally, however, the reverse situation arises in which immobilized biotin in needed to capture avidin or streptavidin components from a sample. These biotin and iminobiotin resins address this special situation.
Immobilized biotin binds avidin and streptavidin very strongly, making it appropriate for non-reversible binding applications. Immobilized iminobiotin is the guanido analog of biotin. The dissociation constant of the avidin-iminobiotin complex is pH dependent. At pH 9.5 to 11, the avidin-iminobiotin complex will bind tightly. At pH 4, the avidin-iminobiotin complex will dissociate. Thus, iminobiotin agarose can be used for situations requiring reversible binding and elution of avidin components.
- Enable purification or removal of avidin or streptavidin samples
- Resins are crosslinked beaded agarose onto which biotin or iminobiotin are covalently immobilized
- Use Biotin Agarose for non-reversible binding (e.g., removal of avidin components from a sample)
- Use Iminobiotin Agarose for reversible binding (e.g., non-denaturing purification of avidin)
|Pierce Iminobiotin Agarose|
|Iminobiotin on crosslinked 6% beaded agarose, slurried in water with sodium azide|
|Binding >/= 1mg of avidin/mL resin|