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Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Resin


Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Manufacturer: thermo scientific™  88273

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Catalog No. PI88273

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Description & Specifications

Specifications

Formulation 5mL-capacity centrifuge columns containing 0.25mL resin
Quantity 5 columns
Sufficient For Removing at least 99.9% of the endotoxin present in typical 0.5 to 1mL samples
Support Type Cellulose
Description Pierce High Capacity Endotoxin Removal Spin Columns, 0.25mL

Reduce endotoxin levels in protein samples by greater than 99% in one hour while maximizing protein recovery with Thermo Scientific™ Pierce High Capacity Endotoxin Removal Resin.

The Pierce High Capacity Endotoxin Removal Resin combines porous cellulose beads and an FDA-approved food preservative, poly(epsilon-lysine), as an affinity ligand to selectively bind endotoxins. Endotoxin levels in protein samples are reduced by greater than 99% in as little as 1 hour using our spin cup format, and protein recovery is ≥85%. The modified polylysine affinity ligand eliminates the toxicity concerns associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers. Pierce High capacity Endotoxin Removal Resin is available as a slurry to pack a custom column that can be re-used at least 5 times with no loss in endotoxin removal efficiency. This resin is also available in a new single-use spin column format optimized for different sample volumes.

Highlights:

  • High capacity bind up to 2,000,000 EU/mL to eliminate at least 99% of endotoxins

  • Durable re-use resin up to 10 times with no loss in performance

  • Selective recover at least 85% of your protein sample

  • High performance compatible with FDA guidelines by reducing final EU concentration to less than 5 EU/mL

  • Fast our new spin cup format enables endotoxin depletion within 1 hour

  • Cleansingle-use spin columns avoid cross contamination of samples

  • Optimized spin cups are optimized for different sample volumes

  • Economical large-volume discounts available

Requires:

Endotoxin-free water

Recommended for:

Eliminate endotoxins from protein or antibody samples prior to cell transfection or animal injection